Coskun Z K, Kerem M, Gurbuz N, Omeroglu S, Pasaoglu H, Demirtas C, Lortlar N, Salman B, Pasaoglu O T, Turgut H B
Department of Anatomy, Gazi University, Faculty of Medicine, Ankara, Turkey.
Bratisl Lek Listy. 2011;112(5):235-43.
To evaluate the beneficial effects of spirulina on the treatment of experimental colitis.
Spirulina, a planktonic blue green algae from oascillateriaceae family, has anti-inflammatory, antioxidant, antitumor, anti-viral, and antimicrobial effects, rendering it a natural drug of prophylactic and therapeutic properties. The effects of spirulina on colitis are not known.
Wistar rats weighing 200-300 g were used. Experimental colitis was created during anesthesia using the trinitrobenzene sulfonic (TNBS) acid. The rats were randomly divided into the 3 groups. In the group 1 (sham; n = 8), saline was administered via oral gavage 7 days after 1 ml of rectal saline was administered. In the group 2 (experimental colitis + spirulina; n = 8), 2 g/kg spirulina was administered via oral gavage 7 days after the rectal 1 ml TNBS was administered. In group 3 (experimental colitis; n = 8), enema was administered via oral gavage 7 days after the rectal 1 ml TNBS was administered. Eight days after the instigation of TNBS colitis, the rats were sacrificed and blood and tissue samples were taken. Histopathologic and immunohistochemical evaluations were conducted, and malondialdehyde (MDA), advanced oxidation protein products (AOPP), catalase (CAT), total antioxidant status (TAS), and glutathione (GSH) levels were determined.
Inflammation on mucosa and submucosa, hemorrhage, necrosis, cellular infiltration and crypt abscess formation, immunoreactivity and tissue MDA levels were decreased in the experimental colitis + spirulina group when compared to the experimental colitis group (p < 0.05).
The results of the present study indicate the beneficial effects of spirulina on TNBS-induced inflammatory bowel disease (Tab. 6, Fig. 10, Ref. 40).
评估螺旋藻对实验性结肠炎治疗的有益效果。
螺旋藻是一种来自颤藻科的浮游蓝绿藻，具有抗炎、抗氧化、抗肿瘤、抗病毒和抗菌作用，使其成为一种具有预防和治疗特性的天然药物。螺旋藻对结肠炎的影响尚不清楚。
使用体重200 - 300克的Wistar大鼠。在麻醉期间用三硝基苯磺酸（TNBS）酸诱导实验性结肠炎。将大鼠随机分为3组。第1组（假手术组；n = 8），在直肠注入1毫升生理盐水7天后，经口灌胃给予生理盐水。第2组（实验性结肠炎 + 螺旋藻组；n = 8），在直肠注入1毫升TNBS 7天后，经口灌胃给予2克/千克螺旋藻。第3组（实验性结肠炎组；n = 8），在直肠注入1毫升TNBS 7天后，经口灌胃给予灌肠剂。在诱导TNBS结肠炎8天后，处死大鼠并采集血液和组织样本。进行组织病理学和免疫组织化学评估，并测定丙二醛（MDA）、晚期氧化蛋白产物（AOPP）、过氧化氢酶（CAT）、总抗氧化状态（TAS）和谷胱甘肽（GSH）水平。
与实验性结肠炎组相比，实验性结肠炎 + 螺旋藻组的黏膜和黏膜下层炎症、出血、坏死、细胞浸润和隐窝脓肿形成、免疫反应性及组织MDA水平均降低（p < 0.05）。
本研究结果表明螺旋藻对TNBS诱导的炎症性肠病具有有益效果（表6，图10，参考文献40）。
