Aziez Meriem, Suharoschi Ramona, Merakeb Mohamed Sofiane, Pop Oana Lelia, Ciont Călina, Ranga Floricuța, Ferhat Riad, Affenai Safia, Vodnar Dan C, Cozma Angela, Fodor Adriana, Mansouri Elhadia, Smati Dalila, Bribi Noureddine
Laboratory of Plant Biotechnology and Ethnobotany, Faculty of Nature and Life Sciences, University of Bejaia, Bejaia 06000, Algeria.
Molecular Nutrition and Proteomics Lab, Bld. Life Science Institute, Department of Food Science, University of Agricultural Science and Veterinary Medicine, 3-5 Calea Mănăstur, 400372 Cluj-Napoca, Romania.
Int J Mol Sci. 2025 Jun 13;26(12):5692. doi: 10.3390/ijms26125692.
, a filamentous photosynthetic cyanobacterium, is widely recognized for its high nutritional value, broad spectrum of bioactive compounds, and excellent safety profile, making it a promising natural source for health-promoting applications. This study aimed to profile the phenolic constituents of an ethanolic extract of (EAP) using HPLC-DAD-ESI-MS and to investigate its pharmacological effects in attenuating acute and sub-chronic experimental colitis, as well as its antioxidant and antifungal properties. Colitis was induced in BALB/c mice by intrarectal administration of 2,4-dinitrobenzenesulfonic acid (DNBS), followed by oral administration of EAP at doses of 50, 100, and 200 mg/kg. Phenolic profiling revealed eight major compounds, with a cumulative content of 6.777 mg/g of extract, with Pyrogallol, Ferulic acid, and Chlorogenic acid being the most abundant. In vivo, EAP treatment significantly reduced the Disease Activity Index (DAI), alleviated macroscopic colonic damage, and preserved colonic mucosal integrity in both inflammatory phases. Biochemical analyses revealed significant reductions in myeloperoxidase (MPO) activity, nitric oxide (NO), and malondialdehyde (MDA) levels, accompanied by increased reduced glutathione (GSH) content and catalase activity. In vitro, EAP demonstrated notable antioxidant effects, including 56% DPPH and 47% ABTS radical scavenging activities, and an 81% ferrous ion-chelating capacity. Furthermore, it exhibited antifungal activity, with inhibition zones of 20 mm against and 15 mm against , respectively. These findings highlight the multitarget bioactivity of EAP and support its potential as a natural agent for managing intestinal inflammation and oxidative stress across both acute and sub-chronic phases.
丝状光合蓝细菌因其高营养价值、广泛的生物活性化合物谱和出色的安全性而被广泛认可，使其成为促进健康应用的有前途的天然来源。本研究旨在使用HPLC-DAD-ESI-MS分析[具体蓝细菌名称未给出]乙醇提取物（EAP）中的酚类成分，并研究其在减轻急性和亚慢性实验性结肠炎方面的药理作用，以及其抗氧化和抗真菌特性。通过直肠内给予2,4-二硝基苯磺酸（DNBS）在BALB/c小鼠中诱导结肠炎，然后以50、100和200 mg/kg的剂量口服EAP。酚类分析揭示了八种主要化合物，提取物中累积含量为6.777 mg/g，其中焦性没食子酸、阿魏酸和绿原酸含量最高。在体内，EAP治疗在两个炎症阶段均显著降低疾病活动指数（DAI），减轻结肠宏观损伤，并保持结肠黏膜完整性。生化分析显示髓过氧化物酶（MPO）活性、一氧化氮（NO）和丙二醛（MDA）水平显著降低，同时还原型谷胱甘肽（GSH）含量和过氧化氢酶活性增加。在体外，EAP表现出显著的抗氧化作用，包括56%的DPPH和47%的ABTS自由基清除活性，以及81%的亚铁离子螯合能力。此外，它还表现出抗真菌活性，对[具体真菌名称未给出1]的抑菌圈为20 mm，对[具体真菌名称未给出2]的抑菌圈为15 mm。这些发现突出了EAP的多靶点生物活性，并支持其作为一种天然药物在急性和亚慢性阶段管理肠道炎症和氧化应激的潜力。
