Gniadek Agnieszka, Macura Anna B
Department of Medical and Environmental Nursing, Faculty of Health Sciences Jagiellonian University Medical College, Kraków, Poland.
Wiad Parazytol. 2011;57(2):103-6.
Infections related to modern surgical procedures present a difficult problem for contemporary medicine. Infections acquired during surgery represent a risk factor related to therapeutical interventions. Eradication of microorganisms from hospital operating theatre environment may contribute to reduction of infections as the laminar flow air-conditioning considerably reduces the number of microorganisms in the hospital environment. The objective of the study was to evaluate the occurrence of fungi in air-conditioned operating theatre rooms. The study was carried out in one of the hospitals in Krak6w during December 2009. Indoor air samples and imprints from the walls were collected from five operating theatre rooms. A total of fifty indoor air samples were collected with a MAS-100 device, and twenty five imprints from the walls were collected using a Count Tact method. Fungal growth was observed in 48 air samples; the average numbers of fungi were within the range of 5-100 c.f.u. in one cubic metre of the air. Fungi were detected only in four samples of the wall imprints; the number of fungi was 0.01 c.f.u. per one square centimetre of the surface. The mould genus Aspergillus was most frequently isolated, and the species A. fumigatus and A. versicolor were the dominating ones. To ensure microbiological cleanness of hospital operating theatre, the air-conditioning system should be properly maintained. Domination of the Aspergillus fungi in indoor air as well as increase in the number of moulds in the samples taken in evenings (p < 0.05) may suggest that the room decontamination procedures were neglected.
与现代外科手术相关的感染给当代医学带来了难题。手术期间获得的感染是与治疗干预相关的一个风险因素。从医院手术室环境中根除微生物可能有助于减少感染，因为层流空调可大幅减少医院环境中的微生物数量。本研究的目的是评估装有空调的手术室房间内真菌的出现情况。该研究于2009年12月在克拉科夫的一家医院进行。从五个手术室房间采集了室内空气样本和墙壁印记。使用MAS - 100设备共采集了五十个室内空气样本，采用Count Tact方法采集了二十五个墙壁印记。在48个空气样本中观察到真菌生长；每立方米空气中真菌的平均数量在5 - 100 c.f.u.范围内。仅在四个墙壁印记样本中检测到真菌；每平方厘米表面的真菌数量为0.01 c.f.u.。最常分离出曲霉属霉菌，烟曲霉和杂色曲霉是主要的菌种。为确保医院手术室的微生物清洁，应妥善维护空调系统。室内空气中曲霉属真菌占主导以及傍晚采集的样本中霉菌数量增加（p < 0.05）可能表明房间净化程序被忽视了。
