Department of Hepatology, Graduate School of Medicine, Osaka City University Central Clinical Laboratory, Osaka City University Medical School Hospital, Osaka, Japan.
Hepatol Res. 2011 Sep;41(9):897-903. doi: 10.1111/j.1872-034X.2011.00842.x. Epub 2011 Jun 17.
Transient elastography is known as a rapid, objective, and highly reliable technique for staging hepatic fibrosis caused by hepatitis C virus infection; however, the relationship between degree of fibrosis and the collagen deposition or the accumulation of myofibroblasts in non-alcoholic fatty liver disease (NAFLD) remains to be further elucidated.
The subjects were 36 patients with NAFLD who received liver biopsy and liver stiffness measurement using transient elastography. Their clinical data and laboratory values were collected. Morphometric analyses of liver fibrosis indicated by collagen deposition and the relative numbers of myofibroblasts were performed.
Liver stiffness measured by transient elastography correlated with histopathological fibrosis staging of NAFLD determined by Brunt's scoring system (P = 0.000149). The fibrosis staging correlated with the ratios of the Sirius red-positive area (P = 0.000032) and α-smooth muscle actin-positive area (P = 0.000898). Finally, liver stiffness significantly correlated with the ratios of the Sirius red-positive area (r = 0.390, P = 0.0184) and α-smooth muscle actin-positive area (r = 0.333, P = 0.0471).
Liver stiffness measurement by transient elastography is valuable for evaluating fibrotic progression in NAFLD.
瞬时弹性成像技术是一种快速、客观、高度可靠的方法，可用于对丙型肝炎病毒感染引起的肝纤维化进行分期；然而，非酒精性脂肪性肝病（NAFLD）纤维化程度与胶原沉积或肌成纤维细胞积累之间的关系仍需进一步阐明。
本研究纳入了 36 名接受过肝活检和瞬时弹性成像肝硬度测量的 NAFLD 患者，收集了他们的临床数据和实验室值。对肝纤维化的胶原沉积和肌成纤维细胞相对数量进行了形态计量学分析。
瞬时弹性成像测量的肝硬度与 Brunt 评分系统确定的 NAFLD 组织学纤维化分期呈显著相关（P=0.000149）。纤维化分期与天狼星红阳性面积比（P=0.000032）和α-平滑肌肌动蛋白阳性面积比（P=0.000898）相关。最后，肝硬度与天狼星红阳性面积比（r=0.390，P=0.0184）和α-平滑肌肌动蛋白阳性面积比（r=0.333，P=0.0471）均呈显著相关。
瞬时弹性成像测量的肝硬度在评估 NAFLD 纤维化进展方面具有重要价值。
