盐酸与硫酸在水相中用于抗酸杆菌的齐-尼染色。

Hydrochloric vs. sulphuric acid in water for Ziehl-Neelsen staining of acid-fast bacilli.

机构信息

Damien Foundation Bangladesh, Dhaka, Bangladesh.

出版信息

Int J Tuberc Lung Dis. 2011 Jul;15(7):955-8. doi: 10.5588/ijtld.10.0722.

DOI:10.5588/ijtld.10.0722

PMID:21682971

Abstract

SETTING

Damien Foundation Bangladesh tuberculosis (TB) control projects.

OBJECTIVES

To compare 25% sulphuric acid in water (H(2)SO(4)) with hydrochloric acid in water (HCl) to differentiate acid-fast bacilli in sputum smears stained with 1% carbolfuchsin.

DESIGN

For 1 year, all 158 microscopy laboratories used either H(2)SO(4) or 3%/6%/10% HCl for their routine work, alternating monthly between H(2)SO(4) and HCl. Each month a sample of five smears per laboratory was rechecked blind. After recording qualitative staining aspects, all sample smears were restained before rechecking, using H(2)SO(4) for destaining.

RESULTS

A total of 368,059 H(2)SO(4) and 335,436 HCl smears were routinely read, yielding 7.2% positive or scanty results in both groups. Of these, 9492 were rechecked. There was no difference in false-negatives detected (0.66%, 95%CI 0.44-0.95 for H(2)SO(4) vs. 0.68%, 95%CI 0.46-0.98 for HCl), but apparently there were more false-positives with H(2)SO(4) (2.12%, 95%CI 0.92-4.14 vs. 0.28%, 95%CI 0.00-1.54, P = 0.05). Qualitatively, only 3% HCl yielded significantly inferior differentiation results.

CONCLUSIONS

HCl 6-10% in water can be recommended for Ziehl-Neelsen destaining above H(2)SO(4). Diluting is easier and safer, and it may cause less confusion with false-positives during rechecking, including a restaining step.

摘要

背景

达米恩基金会孟加拉国结核病（TB）控制项目。

目的

比较水 25%硫酸（H2SO4）和盐酸（HCl）在 1%咔红染色的痰涂片上区分抗酸杆菌的效果。

设计

在 1 年的时间里，所有 158 个显微镜实验室都使用 H2SO4 或 3%/6%/10% HCl 进行常规工作，每月在 H2SO4 和 HCl 之间交替。每个月每个实验室检查 5 张涂片样本，进行盲法复查。记录定性染色情况后，所有样本涂片都用 H2SO4 进行脱染，然后重新用 H2SO4 进行染色，进行复查。

结果

共常规读取了 368059 张 H2SO4 和 335436 张 HCl 涂片，两组的阳性或少量结果均为 7.2%。其中 9492 张进行了复查。未发现假阴性检测差异（H2SO4 为 0.66%，95%CI 0.44-0.95；HCl 为 0.68%，95%CI 0.46-0.98），但 H2SO4 的假阳性似乎更多（2.12%，95%CI 0.92-4.14；HCl 为 0.28%，95%CI 0.00-1.54，P = 0.05）。定性分析显示，只有 3% HCl 的区分效果明显较差。