Department of Analytical Chemistry, Faculty of Chemistry, University of Alcalá, Alcalá de Henares, Madrid, Spain.
J Chromatogr A. 2011 Jul 29;1218(30):4928-33. doi: 10.1016/j.chroma.2011.05.055. Epub 2011 May 23.
The determination of bioactive peptides derived from food sources is gaining special attention in last years, due to their ability to promote health and their potential to reduce the risk of chronic diseases. In this work, a new analytical methodology has been developed enabling for the first time the determination of soymetide, a new immunostimulating peptide derived from soybean, in different soybean derived foodstuffs. Capillary-HPLC was employed for peptide separation after accelerated tryptic digestion of soybean proteins. Two different capillary-HPLC columns were tested and chromatographic separation was optimized in each case. The use of a 300 μm fused-core technology C18 column enabled a suitable separation of soymetide from the other peptides in less than 18 min. Different analytical characteristics of the method were evaluated: selectivity, linearity, accuracy, precision, limit of detection and quantitation, and stability. The developed method was applied to the determination of soymetide content in different soybean dairy-like products for human consumption (powdered milks and infant formulas).
近年来，由于生物活性肽具有促进健康和降低慢性病风险的潜力，因此源自食物来源的生物活性肽的测定受到了特别关注。在这项工作中，开发了一种新的分析方法，首次能够在不同的大豆衍生食品中测定源自大豆的新型免疫刺激肽——soymetide。在加速大豆蛋白的胰蛋白酶消化后，采用毛细管 HPLC 进行肽分离。测试了两种不同的毛细管 HPLC 柱，并在每种情况下对色谱分离进行了优化。使用 300μm 熔融核技术 C18 柱，能够在不到 18 分钟的时间内将 soymetide 与其他肽适当地分离。评估了该方法的不同分析特性：选择性、线性、准确性、精密度、检测限和定量限以及稳定性。该方法已应用于测定不同供人类食用的大豆乳制品（奶粉和婴儿配方奶粉）中 soymetide 的含量。
