Centro para la Calidad de los Alimentos, Instituto Nacional de Investigación Y Tecnología Agraria y Alimentaria (INIA), C/José Tudela s/n, 42004 Soria, Spain.
Talanta. 2010 Oct 15;82(5):1986-94. doi: 10.1016/j.talanta.2010.08.050. Epub 2010 Sep 17.
The recent development of fused-core technology in HPLC columns is enabling faster and highly efficient separations. This technology was evaluated for the development of an fast analysis method for the most relevant soy isoflavones. A step-by-step strategy was used to optimize temperature (25-50°C), flow rate (1.2-2.7 mL/min), mobile phase composition and equilibration time (1-5 min). Optimized conditions provided a method for the separation of all isoflavones in less than 5.8 min and total analysis time (sample-to-sample) of 11.5 min. Evaluation of chromatographic performance revealed excellent reproducibility, resolution, selectivity, peak symmetry and low limits of detection and quantification levels. The use of a fused-core column allows highly efficient, sensitive, accurate and reproducible determination of isoflavones with an outstanding sample throughout and resolution. The developed method was validated with different soy samples with a total isoflavone concentration ranging from 1941.53 to 2460.84 μg g(-1) with the predominant isoflavones being isoflavone glucosides and malonyl derivatives.
高效核壳技术在 HPLC 柱中的最新发展使得快速且高效的分离成为可能。本研究采用该技术开发了一种快速分析方法，用于检测最相关的大豆异黄酮。采用逐步优化策略对温度（25-50°C）、流速（1.2-2.7mL/min）、流动相组成和平衡时间（1-5min）进行了优化。优化条件下，所有异黄酮在不到 5.8min 内得到分离，总分析时间（样品到样品）为 11.5min。色谱性能评价结果表明，该方法具有良好的重现性、分辨率、选择性、峰对称性和低检测限和定量限。高效核壳柱的使用可实现异黄酮的高效、灵敏、准确和重现性测定，具有出色的样品通量和分辨率。该方法已用于不同大豆样品的验证，总异黄酮浓度范围为 1941.53-2460.84μg/g，主要异黄酮为异黄酮糖苷和丙二酰衍生物。
