Department of Urology, University of Toledo Medical Center, Toledo, Ohio 43614, USA.
J Urol. 2011 Aug;186(2):702-6. doi: 10.1016/j.juro.2011.03.125. Epub 2011 Jun 17.
Mitomycin C (Novaplus®) is often instilled intravesically in the postoperative period to prevent tumor cell implantation/regrowth after transurethral tumor resection. In an earlier study EGCG prevented tumor cell implantation/growth in an experimental bladder tumor model simulating clinical transurethral bladder resection. We compared the efficacy of EGCG (Polyphenon E®) to that of mitomycin C to prevent tumor cell implantation/growth in this model.
Mitomycin C and EGCG were studied for their in vitro and in vivo effects. The AY-27 rat urothelial tumor cell line was used for in vitro and in vivo studies. In vitro cell viability studies included trypan blue exclusion, MTT proliferation assay and clonal growth assay. Fischer 344 female rats were used for intravesical tumor implantation/growth assay using an electrocautery injury model. Tumor growth in vivo was assessed in controls treated with phosphate buffered saline and in bladders treated with mitomycin C or EGCG by standard histological techniques using hematoxylin and eosin 4 weeks after injury.
Mitomycin C and EGCG showed cytotoxicity on all in vitro assays. They were equivalent for preventing intravesical tumor growth.
EGCG prevents intravesical tumor growth with efficacy equivalent to that of mitomycin C in this experimental model.
丝裂霉素 C（Novaplus®）常在经尿道肿瘤切除术后膀胱内灌注，以防止肿瘤细胞种植/复发。在早期的一项研究中，EGCG 在模拟临床经尿道膀胱切除术的实验性膀胱肿瘤模型中预防肿瘤细胞种植/生长。我们比较了 EGCG（多酚 E®）和丝裂霉素 C 预防该模型中肿瘤细胞种植/生长的效果。
研究了丝裂霉素 C 和 EGCG 的体外和体内作用。使用 AY-27 大鼠尿路上皮肿瘤细胞系进行体外和体内研究。体外细胞活力研究包括台盼蓝排斥试验、MTT 增殖试验和克隆生长试验。使用电灼损伤模型，对 Fischer 344 雌性大鼠进行膀胱内肿瘤种植/生长试验。用磷酸盐缓冲盐水处理对照组，用丝裂霉素 C 或 EGCG 处理膀胱，4 周后用苏木精和伊红进行标准组织学技术评估体内肿瘤生长。
丝裂霉素 C 和 EGCG 在所有体外试验中均显示细胞毒性。它们在预防膀胱内肿瘤生长方面效果相当。
在该实验模型中，EGCG 可有效预防膀胱内肿瘤生长，与丝裂霉素 C 相当。
