Newman S L, Tucci M A
Department of Internal Medicine, University of Cincinnati Medical Center, Ohio 45267.
J Clin Invest. 1990 Sep;86(3):703-14. doi: 10.1172/JCI114766.
In inflammation monocytes emigrate from the peripheral circulation into an extravascular area rich in extracellular matrix proteins. In this milieu, phagocytes ingest and kill invading pathogens. In the present studies, we found that monocytes adhered to type I collagen gels phagocytized 2.5-12-fold more opsonized Escherichia coli, Staphylococcus aureus, Streptococcus pyogenes, and Streptococcus pneumoniae than plastic-adherent monocytes. The rate of phagocytosis and the number of bacteria ingested by collagen-adherent monocytes was equal to, or greater than, the number of bacteria ingested by 7-d cultured macrophages (M phi). Although both collagen- and plastic-adherent monocytes were bactericidal for E. coli and S. aureus, more bacteria were killed by collagen-adherent monocytes by virtue of their enhanced phagocytic capacity. Cultured M phi only were bacteriostatic. Adherence of monocytes to collagen gels activated C receptors (CR) types 1 and 3 for phagocytosis, and enhanced Fc receptor (FcR)-mediated phagocytosis. Collagen- and plastic-adherent monocytes produced equivalent amounts of superoxide anion in response to phorbol myristate acetate and opsonized zymosan. Thus, the enhanced phagocytosis and killing of opsonized bacteria by collagen-adherent monocytes appear to be by regulation of the function of membrane CR and FcR, without apparent enhancement of the respiratory burst. These data suggest that adherence of monocytes to the extracellular matrix during inflammation may rapidly activate these cells for enhanced phagocytic bactericidal activity.
在炎症过程中，单核细胞从外周循环迁移至富含细胞外基质蛋白的血管外区域。在这种环境中，吞噬细胞摄取并杀死入侵的病原体。在本研究中，我们发现，与黏附于塑料的单核细胞相比，黏附于I型胶原凝胶的单核细胞吞噬调理过的大肠杆菌、金黄色葡萄球菌、化脓性链球菌和肺炎链球菌的能力要强2.5至12倍。胶原黏附单核细胞的吞噬速率及摄取的细菌数量等于或大于7天培养的巨噬细胞摄取的细菌数量。虽然胶原黏附单核细胞和塑料黏附单核细胞对大肠杆菌和金黄色葡萄球菌均有杀菌作用，但胶原黏附单核细胞因其增强的吞噬能力而能杀死更多细菌。培养的巨噬细胞仅具有抑菌作用。单核细胞黏附于胶原凝胶可激活1型和3型吞噬C受体（CR），并增强Fc受体（FcR）介导的吞噬作用。胶原黏附单核细胞和塑料黏附单核细胞对佛波酯肉豆蔻酸酯乙酸盐和调理过的酵母聚糖产生等量的超氧阴离子。因此，胶原黏附单核细胞对调理过细菌的吞噬和杀伤作用增强似乎是通过调节膜CR和FcR的功能实现的，而呼吸爆发无明显增强。这些数据表明，炎症期间单核细胞黏附于细胞外基质可能会迅速激活这些细胞，以增强吞噬杀菌活性。
