Newman S L, Musson R A, Henson P M
J Immunol. 1980 Nov;125(5):2236-44.
The development of Fc and C3 receptor function was evaluated during differentiation of human peripheral blood monocytes into macrophages in an in vitro culture system. At the time of isolation, monocytes formed rosettes with C3-coated erythrocytes (EIgMC) but did nt internalize them, whereas IgG-coated erythrocytes (EIgG) were both bound and ingested. During the first 24 hr in culture, IgG-mediated phagocytosis and C3-mediated rosette formation declined. After 7 days of in vitro differentiation, monocyte-derived macrophages were capable of ingesting 2 to 4 times as many EIgG as uncultured monocytes. In addition, macrophages demonstrated a new function--the ability to ingest C3-coated erythrocytes, as well as to efficiently bind them. The time course of the reappearance of receptor functions varied between different individuals, but by day 7, the activity of Fc and C3 receptors was vigorous for all individuals tested. Monocytes underwent differentiation whether they were cultured on glass coverslips or on a plastic surface. However, macrophages demonstrated C3-mediated ingestion earlier when cultured on plastic. Culturing monocytes on the different surfaces did not affect the time course of the reappearance of Fc receptor function, but did have a small effect on the magnitude of the response. These experiments demonstrate for the first time, that a defined population of human phagocytic cells can acquire the ability to mediate ingestion through their C3 receptors.
在体外培养系统中，研究了人外周血单核细胞分化为巨噬细胞过程中Fc和C3受体功能的发育情况。分离时，单核细胞能与C3包被的红细胞（EIgMC）形成玫瑰花结，但不将其内化，而IgG包被的红细胞（EIgG）既能结合又能被摄取。在培养的最初24小时内，IgG介导的吞噬作用和C3介导的玫瑰花结形成减少。体外分化7天后，单核细胞衍生的巨噬细胞摄取EIgG的能力是未培养单核细胞的2至4倍。此外，巨噬细胞表现出一种新功能——摄取C3包被红细胞的能力，以及有效结合它们的能力。受体功能重新出现的时间进程在不同个体之间有所不同，但到第7天，所有测试个体的Fc和C3受体活性都很强。单核细胞无论在玻璃盖玻片上还是在塑料表面培养都会发生分化。然而，在塑料上培养时，巨噬细胞更早地表现出C3介导的摄取。在不同表面培养单核细胞不影响Fc受体功能重新出现的时间进程，但对反应的强度有轻微影响。这些实验首次证明，特定群体的人类吞噬细胞能够获得通过其C3受体介导摄取的能力。
