人 U2B″ 蛋白与 snRNA 茎环的结合。
Human U2B″ protein binding to snRNA stemloops.
机构信息
Department of Biochemistry and Molecular Biophysics, Washington University Medical School, St Louis, MO 63110, United States.
出版信息
Biophys Chem. 2011 Nov;159(1):82-9. doi: 10.1016/j.bpc.2011.05.010. Epub 2011 May 16.
Abstract
The human U2B″ protein is one of the unique proteins that comprise the U2 snRNP, but it is also a representative of the U1A/U2B″ protein family. In the U2 snRNP, it is bound to Stem-Loop IV (SLIV) of the U2 snRNA. We find that in vitro it binds not only to human SLIV, but also to Stem-Loop II (SLII) from human U1 snRNA and to Drosophila U2 snRNA SLIV. The thermodynamics of these binding interactions show a striking similarity, leading to the conclusion that U2B″ has a relaxed specificity for its RNA targets. The binding properties of U2B″ are distinct from those of human U1A and of Drosophila SNF, despite its high homology to those proteins, and so provide important new information on how this protein family has modulated its target preferences.
摘要
人类 U2B″ 蛋白是构成 U2 snRNP 的独特蛋白之一，但它也是 U1A/U2B″ 蛋白家族的代表。在 U2 snRNP 中，它与 U2 snRNA 的茎环 IV（SLIV）结合。我们发现，它不仅在体外与人 SLIV 结合，还与人 U1 snRNA 的茎环 II（SLII）和果蝇 U2 snRNA SLIV 结合。这些结合相互作用的热力学性质非常相似，得出结论，U2B″ 对其 RNA 靶标具有宽松的特异性。尽管 U2B″ 与人类 U1A 和果蝇 SNF 具有高度同源性，但它的结合特性与这些蛋白质明显不同，因此为了解该蛋白质家族如何调节其靶标偏好提供了重要的新信息。
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