UGC-Centre for Advanced Studies, University Institute of Pharmaceutical Sciences, Panjab University, Chandigarh-160014, India.
Acta Pharm. 2011 Jun;61(2):141-56. doi: 10.2478/v10007-011-0012-8.
Bovine serum albumin (BSA) nanoparticles loaded with paclitaxel (PTX) were prepared using a desolvation technique. A 32 full factorial design (FFD) was employed to formulate nanoparticles. Nanoparticles were characterized for particle size by photon correlation spectroscopy and surface morphology by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Encapsulation efficiency, zeta potential and particle yield were also determined. Response surface linear modelling (RSLM) was used to predict the optimal formulation. Various models were applied to determine the release mechanism from PTX nanoparticles. The effect of drug-polymer ratio on the release profile of formulations was observed and was applied to determine the suitability of the predicted optimal formulation. A preliminary study to determine the feasibility of targeting the prepared nanoparticles to brain was also carried out using mice as in vivo models.
牛血清白蛋白（BSA）载紫杉醇（PTX）纳米粒采用相分离技术制备。采用 32 全因子设计（FFD）来制备纳米粒。用光子相关光谱法测定纳米粒的粒径，用扫描电子显微镜（SEM）和透射电子显微镜（TEM）观察纳米粒的表面形态。测定包封率、Zeta 电位和纳米粒得率。采用响应面线性建模（RSLM）预测最佳处方。采用各种模型来确定 PTX 纳米粒的释放机制。观察药物-聚合物比对制剂释放特征的影响，并用于确定预测的最佳处方的适用性。还使用小鼠作为体内模型进行了初步研究，以确定制备的纳米粒靶向大脑的可行性。
