Yadav Ramdhan, Kumar Dharmesh, Kumari Avnesh, Yadav Sudesh Kumar
Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology, India ; Academy of Scientific and Innovative Research (AcSIR), New Delhi.
Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology, India.
EXCLI J. 2014 Apr 1;13:331-46. eCollection 2014.
Nanoencapsulation of antioxidant molecules on protein nanoparticles (NPs) could be an advanced approach for providing stable, better food nutraceuticals and anticancer drugs. The bioavailability and stability of catechin (CAT) and epicatechin (ECAT) were very poor. In the present study, the CAT and ECAT were loaded on bovine serum albumin (BSA) NPs following desolvation method. The transmission electron microscope (TEM) and atomic force microscope (AFM) recorded size of CAT-BSA NPs and ECAT-BSA NPs were 45 ± 5 nm and 48 ± 5 nm respectively. The encapsulation efficiency of CAT and ECAT on BSA NPs was found to be 60.5 and 54.5 % respectively. CAT-BSA NPs and ECAT-BSA NPs show slow and sustained in vitro release. The CAT-BSA NPs and ECAT-BSA NPs were stable in solution at various temperatures 37 °C, 47 °C and 57 °C. DPPH assay revealed that CAT and ECAT maintained their functional activity even after encapsulation on BSA NPs. Furthermore, the efficacy of CAT-BSA NPs and ECAT-BSA NPs determined against A549 cell lines was found to be improved. CAT and ECAT aptly encapsulated in BSA NPs, showed satisfactory sustained release, maintained antioxidant potential and found improved efficacy. This has thus suggested their more effective use in food and nutraceuticals as well as in medical field.
将抗氧化分子纳米封装在蛋白质纳米颗粒（NPs）上，可能是一种提供稳定、更好的食品营养保健品和抗癌药物的先进方法。儿茶素（CAT）和表儿茶素（ECAT）的生物利用度和稳定性非常差。在本研究中，采用去溶剂法将CAT和ECAT负载在牛血清白蛋白（BSA）纳米颗粒上。透射电子显微镜（TEM）和原子力显微镜（AFM）记录的CAT-BSA纳米颗粒和ECAT-BSA纳米颗粒的尺寸分别为45±5 nm和48±5 nm。发现CAT和ECAT在BSA纳米颗粒上的包封率分别为60.5%和54.5%。CAT-BSA纳米颗粒和ECAT-BSA纳米颗粒显示出缓慢且持续的体外释放。CAT-BSA纳米颗粒和ECAT-BSA纳米颗粒在37℃、47℃和57℃等不同温度的溶液中稳定。DPPH测定表明，CAT和ECAT即使在包封于BSA纳米颗粒后仍保持其功能活性。此外，发现针对A549细胞系测定的CAT-BSA纳米颗粒和ECAT-BSA纳米颗粒的功效有所提高。CAT和ECAT恰当地封装在BSA纳米颗粒中，显示出令人满意的持续释放，保持了抗氧化潜力，并发现功效有所提高。因此，这表明它们在食品和营养保健品以及医学领域有更有效的用途。
