Gu Y, Chang C J, Rikihisa Y, Lin Y C
Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine, Ohio State University, Columbus 43210-1092.
Life Sci. 1990;47(5):407-14. doi: 10.1016/0024-3205(90)90298-6.
Inhibitory effects of gossypol on the female reproductive system have been recently reported. This study investigated a possible site of gossypol action on progesterone synthesis. Bovine luteal cells were cultured with hCG and forskolin in the presence or absence of gossypol. At 10 micrograms/ml, gossypol significantly inhibited hCG- and forskolin-stimulated progesterone secretion and intracellular cAMP formation; at 20 micrograms/ml, gossypol completely abolished the stimulative effect of hCG and forskolin. The results suggest that adenylate cyclase may be a site of gossypol action on steroidogenesis of bovine luteal cells.
最近有报道称棉酚对雌性生殖系统有抑制作用。本研究调查了棉酚对孕酮合成作用的可能位点。在有或无棉酚存在的情况下，将牛黄体细胞与hCG和福司可林一起培养。棉酚浓度为10微克/毫升时，可显著抑制hCG和福司可林刺激的孕酮分泌及细胞内cAMP的形成；浓度为20微克/毫升时，棉酚完全消除了hCG和福司可林的刺激作用。结果表明，腺苷酸环化酶可能是棉酚作用于牛黄体细胞类固醇生成的位点。
