Zheng Y H, Wu Z H, Fang L
Department of Physiology, Jiangxi Medical College, Nanchang.
Yao Xue Xue Bao. 1991;26(11):805-8.
The effect of gossypol acetic acid on hCG-stimulated progesterone synthesis and secretion in isolated rat luteal cells and its possible mechanism were investigated. When gossypol was used at a concentration of 20 micrograms/ml for one hour, the hCG-stimulated progesterone production and secretion decreased markedly, but the basal progesterone level did not change significantly. The cell viability did not decrease after four hours incubation. The inhibition persisted even when cells exposed to gossypol were washed thoroughly with Eagle solution. Gossypol also reduced the cAMP level. The present experiment indicates that gossypol acetic acid can inhibit the progesterone production stimulated by hCG in isolated rat luteal cells through direct cellular mechanism.
研究了醋酸棉酚对离体大鼠黄体细胞中hCG刺激的孕酮合成和分泌的影响及其可能机制。当以20微克/毫升的浓度使用棉酚1小时时，hCG刺激的孕酮产生和分泌明显降低，但基础孕酮水平无明显变化。孵育4小时后细胞活力未降低。即使将暴露于棉酚的细胞用伊格尔溶液彻底洗涤，抑制作用仍持续存在。棉酚还降低了cAMP水平。本实验表明，醋酸棉酚可通过直接细胞机制抑制离体大鼠黄体细胞中hCG刺激的孕酮产生。
