Préat T, Thérond P, Lamour-Isnard C, Limbourg-Bouchon B, Tricoire H, Erk I, Mariol M C, Busson D
Centre de Génétique Moléculaire du CNRS, Université P. et M. Curie, Gif-sur-Yvette, France.
Nature. 1990 Sep 6;347(6288):87-9. doi: 10.1038/347087a0.
The segmented pattern of the Drosophila embryo depends on a regulatory cascade involving three main classes of genes. An early regulatory programme, set up before cellularization, involves direct transcriptional regulation mediated by gap and pair-rule genes. In a second phase occurring after cellularization, interactions between segment-polarity genes are involved in cell communication. Segment-polarity genes are required for pattern formation in different domains of each metamere and act to define and maintain positional information in each segment. The segment-polarity gene fused is maternally required for correct patterning in the posterior part of each embryonic metamere. It is also necessary later in development, as fused mutations lead to anomalies of adult cuticular structures and tumorous ovaries. Here we provide molecular evidence that this gene encodes a putative serine/threonine protein kinase, a new function for the product of a segmentation gene. This result provides further insight into segment-polarity interactions and their role in pattern formation.
果蝇胚胎的分节模式取决于一个涉及三类主要基因的调控级联。在细胞化之前建立的早期调控程序涉及由间隙基因和成对规则基因介导的直接转录调控。在细胞化之后发生的第二阶段，段极性基因之间的相互作用参与细胞通讯。段极性基因是每个体节不同区域模式形成所必需的，并在每个节段中定义和维持位置信息。段极性基因融合对于每个胚胎体节后半部分的正确模式形成是母体所必需的。在发育后期它也是必需的，因为融合突变会导致成虫表皮结构异常和卵巢肿瘤。在这里，我们提供了分子证据，表明该基因编码一种假定的丝氨酸/苏氨酸蛋白激酶，这是一种分节基因产物的新功能。这一结果为段极性相互作用及其在模式形成中的作用提供了进一步的见解。
