Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences/Ministry of Agriculture Key Laboratory of Crop Nutrition and Fertilization, Beijing 100081, PR China.
State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing 100193, PR China.
Int J Syst Evol Microbiol. 2012 May;62(Pt 5):1032-1036. doi: 10.1099/ijs.0.030858-0. Epub 2011 Jun 17.
A novel moderately halophilic bacterium, designated strain K170(T), was isolated from Keke Salt Lake in Qinghai, China. The strain grew with 0-22 % (w/v) NaCl, at 4-50 °C and at pH 6-11, with optimum growth in 3 % (w/v) NaCl, at 40 °C and at pH 8. The predominant respiratory quinone was menaquinone 7 (MK-7). The polar lipids included diphosphatidylglycerol, phosphatidylglycerol, unidentified phospholipids, aminolipids and glycolipids. The major cellular fatty acids were anteiso-C(15 : 0), iso-C(15 : 0) and anteiso-C(17 : 0). The DNA G+C content was 35.8 mol%. Phylogenetic analysis based on the full-length 16S rRNA gene sequence revealed that strain K170(T) was a member of the genus Gracilibacillus. High levels of 16S rRNA gene sequence similarity were found between strain K170(T) and Gracilibacillus boraciitolerans DSM 17256(T) (97.3 %) and Gracilibacillus thailandensis JCM 15569(T) (97.1 %). 16S rRNA gene sequence similarities between strain K170(T) and the type strains of other recognized members of the genus Gracilibacillus were below 97 %. The DNA-DNA hybridization values of strain K170(T) with G. boraciitolerans DSM 17256(T) and G. thailandensis JCM 15569(T) were 21.9 % and 34.3 %, respectively. On the basis of these results, strain K170(T) is considered to represent a novel species of the genus Gracilibacillus, for which the name Gracilibacillus kekensis sp. nov. is proposed; the type strain is K170(T) ( = CGMCC 1.10681(T) = DSM 23178(T)).
一株新型中度嗜盐细菌，命名为 K170(T) 菌株，从中国青海可可盐湖中分离得到。该菌株在 0-22%（w/v）NaCl、4-50°C 和 pH6-11 的条件下生长，最适生长条件为 3%（w/v）NaCl、40°C 和 pH8。主要的呼吸醌是甲萘醌 7（MK-7）。极性脂包括双磷脂酰甘油、磷脂酰甘油、未鉴定的磷脂、胺脂和糖脂。主要的细胞脂肪酸为anteiso-C(15：0)、iso-C(15：0)和anteiso-C(17：0)。DNA G+C 含量为 35.8mol%。基于全长 16S rRNA 基因序列的系统发育分析表明，菌株 K170(T)是 Gracilibacillus 属的一个成员。菌株 K170(T)与 Gracilibacillus boraciitolerans DSM 17256(T)（97.3%）和 Gracilibacillus thailandensis JCM 15569(T)（97.1%）的 16S rRNA 基因序列相似度很高。菌株 K170(T)与其他已确认的 Gracilibacillus 属成员的模式菌株的 16S rRNA 基因序列相似度均低于 97%。菌株 K170(T)与 G. boraciitolerans DSM 17256(T)和 G. thailandensis JCM 15569(T)的 DNA-DNA 杂交值分别为 21.9%和 34.3%。基于这些结果，菌株 K170(T)被认为代表了 Gracilibacillus 属的一个新种，命名为 Gracilibacillus kekensis sp. nov.；其模式菌株为 K170(T)（=CGMCC 1.10681(T)=DSM 23178(T)）。
