Curriculum in Genetics and Molecular Biology, UNC Lineberger Comprehensive Cancer Center, UNC Center for AIDS Research, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7290, USA.
Blood. 2011 Aug 18;118(7):e32-9. doi: 10.1182/blood-2010-12-323659. Epub 2011 Jun 17.
Primary effusion lymphoma (PEL) is a diffuse-large B-cell lymphoma with poor prognosis. One hundred percent of PELs carry the genome of Kaposi sarcoma-associated herpesvirus and a majority are coinfected with Epstein-Barr virus (EBV). We profiled genomic aberrations in PEL cells using the Affymetrix 6.0 SNP array. This identified for the first time individual genes that are altered in PEL cells. Eleven of 13 samples (85%) were deleted for the fragile site tumor suppressors WWOX and FHIT. Alterations were also observed in the DERL1, ETV1, RASA4, TPK1, TRIM56, and VPS41 genes, which are yet to be characterized for their roles in cancer. Coinfection with EBV was associated with significantly fewer gross genomic aberrations, and PEL could be segregated into EBV-positive and EBV-negative clusters on the basis of host chromosome alterations. This suggests a model in which both host genetic aberrations and the 2 viruses contribute to the PEL phenotype.
原发性渗出性淋巴瘤（PEL）是一种预后不良的弥漫性大 B 细胞淋巴瘤。100%的 PEL 携带卡波西肉瘤相关疱疹病毒的基因组，且大多数同时感染 Epstein-Barr 病毒（EBV）。我们使用 Affymetrix 6.0 SNP 芯片对 PEL 细胞中的基因组异常进行了分析。这是首次确定 PEL 细胞中发生改变的个别基因。13 个样本中的 11 个（85%）缺失脆性部位肿瘤抑制基因 WW0X 和 FHIT。在 DERL1、ETV1、RASA4、TPK1、TRIM56 和 VPS41 基因中也观察到了改变，这些基因在癌症中的作用尚待研究。与 EBV 共同感染与明显较少的总体基因组异常相关，并且可以根据宿主染色体改变将 PEL 分为 EBV 阳性和 EBV 阴性簇。这表明，宿主遗传异常和这两种病毒都促成了 PEL 表型。
