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质粒RP4转移起始点处松弛体的体外组装。

In vitro assembly of relaxosomes at the transfer origin of plasmid RP4.

作者信息

Pansegrau W, Balzer D, Kruft V, Lurz R, Lanka E

机构信息

Max-Planck-Institut für Molekulare Genetik, Abteilung Schuster, Berlin, Federal Republic of Germany.

出版信息

Proc Natl Acad Sci U S A. 1990 Sep;87(17):6555-9. doi: 10.1073/pnas.87.17.6555.

DOI:10.1073/pnas.87.17.6555
PMID:2168553
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC54575/
Abstract

During initiation of conjugative transfer of DNA containing the transfer origin (oriT) of the promiscuous plasmid RP4, the proteins TraI, TraJ, and TraH interact and assemble a specialized nucleoprotein complex (the relaxosome) at oriT. The structure can be visualized on electron micrographs. Site- and strand-specific nicking at the transfer origin in vitro is dependent on the proteins TraI and TraJ and on Mg2+ ions. Substrate specificity is directed exclusively towards the cognate transfer origin: the RP4-specified TraJ protein cannot recognize the closely related oriT of plasmid R751. After nicking, TraI protein remains attached to the 5'-terminal 2'-deoxycytidyl residue at the nic site [Pansegrau, W., Ziegelin, G. & Lanka, E. (1990) J. Biol. Chem. 265, 10637-10644]. Nicking and relaxosome formation require supercoiled DNA. Thus, a complicated structure involving multiple plasmid-specified proteins and a defined region of DNA must be formed at the transfer origin to prepare the plasmid for generating the single strand to be transferred.

摘要

在含有滥交质粒RP4转移起始点（oriT）的DNA进行接合转移起始过程中，TraI、TraJ和TraH蛋白相互作用，并在oriT处组装一个特殊的核蛋白复合体（松弛体）。该结构可在电子显微镜照片上观察到。体外转移起始点处的位点特异性和链特异性切口依赖于TraI和TraJ蛋白以及Mg2+离子。底物特异性仅针对同源转移起始点：RP4指定的TraJ蛋白无法识别质粒R751密切相关的oriT。切口后，TraI蛋白仍附着在切口位点的5'-末端2'-脱氧胞苷残基上[潘塞格劳，W.，齐格林，G. & 兰卡，E.（1990年）《生物化学杂志》265，10637 - 10644]。切口和松弛体形成需要超螺旋DNA。因此，必须在转移起始点形成一个涉及多种质粒指定蛋白和特定DNA区域的复杂结构，以便为质粒生成待转移的单链做好准备。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e047/54575/115d5315d5f4/pnas01042-0092-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e047/54575/c6f6dc097a07/pnas01042-0090-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e047/54575/9149a21c8838/pnas01042-0091-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e047/54575/08b40355dcca/pnas01042-0091-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e047/54575/9e1d30bf806f/pnas01042-0091-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e047/54575/10171ec75c10/pnas01042-0092-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e047/54575/115d5315d5f4/pnas01042-0092-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e047/54575/c6f6dc097a07/pnas01042-0090-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e047/54575/9149a21c8838/pnas01042-0091-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e047/54575/08b40355dcca/pnas01042-0091-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e047/54575/9e1d30bf806f/pnas01042-0091-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e047/54575/10171ec75c10/pnas01042-0092-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e047/54575/115d5315d5f4/pnas01042-0092-b.jpg

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2
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J Mol Biol. 1983 Jun 5;166(4):557-80. doi: 10.1016/s0022-2836(83)80284-8.
3
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mSphere. 2020 Aug 26;5(4):e00748-20. doi: 10.1128/mSphere.00748-20.
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