Centre for Research in Infectious Diseases, School of Medicine and Medical Science, University College Dublin, Belfield, Dublin, Ireland.
Transplantation. 2011 Aug 15;92(3):328-33. doi: 10.1097/TP.0b013e3182247bf2.
Studies have identified solid organ transplant recipients who remain asymptomatic despite maintaining chronic high Epstein-Barr virus (EBV) viral loads. We examined clinical manifestations, EBV gene expression, human leukocyte antigen (HLA) alleles, and specific T-cell responses to EBV infection in pediatric renal transplant patients.
Seventeen pediatric renal transplant patients were categorized according to EBV viral load into those with chronic high viral loads (CHL) and recipients who resolve EBV infection (REI). EBV gene expression was analyzed using real-time PCR assays and EBV-specific T cells were analyzed by flow cytometry.
EBV gene, EBV-encoded small RNA 1, was expressed at significantly higher levels in CHL compared with EBV seropositive controls (P=0.005) and raised compared with REI. BamHI A right-ward transcripts were also expressed at higher levels in CHL patients (P=0.03) than in REI. Expression of latent genes, EBNA1, LMP1, LMP2, and lytic gene BZLF1 were restricted to the CHL group with viral gene expression varying over time. HLA-A02 allele expression was predominant in CHL patients (80%) and GLC lytic-specific cytotoxic T-lymphocytes were absent. In contrast, HLA-B08 allele expression was prevalent in REI patients (71%) and RAK lytic cytotoxic T-lymphocytes were detected in all patients.
EBV gene expression in CHL carriers differs from those that resolve infection and should be interpreted alongside HLA polymorphisms.
研究已经确定了一些实体器官移植受者，尽管他们持续存在慢性高 Epstein-Barr 病毒 (EBV) 病毒载量，但仍无症状。我们检查了儿科肾移植患者的临床表现、EBV 基因表达、人类白细胞抗原 (HLA) 等位基因和 EBV 感染的特异性 T 细胞反应。
根据 EBV 病毒载量，将 17 名儿科肾移植患者分为慢性高病毒载量 (CHL) 组和 EBV 感染消退 (REI) 组。使用实时 PCR 检测 EBV 基因表达，通过流式细胞术分析 EBV 特异性 T 细胞。
与 EBV 血清阳性对照相比，CHL 中 EBV 基因，即 EBV 编码的小 RNA 1 的表达水平显著升高 (P=0.005)，与 REI 相比也升高。BamHI A 右向转录物在 CHL 患者中的表达水平也高于 REI (P=0.03)。潜伏基因 EBNA1、LMP1、LMP2 和裂解基因 BZLF1 的表达仅限于 CHL 组，病毒基因表达随时间变化。CHL 患者 HLA-A02 等位基因表达为主 (80%)，而 GLC 裂解特异性细胞毒性 T 淋巴细胞缺失。相比之下，REI 患者 HLA-B08 等位基因表达更为普遍 (71%)，并且在所有患者中均检测到 RAK 裂解细胞毒性 T 淋巴细胞。
CHL 携带者的 EBV 基因表达与那些清除感染的人不同，应与 HLA 多态性一起解释。
