Department of Virology, Nagoya University Graduate School of Medicine, Nagoya, Japan.
J Gen Virol. 2010 Jan;91(Pt 1):42-50. doi: 10.1099/vir.0.013482-0. Epub 2009 Sep 30.
Chronic active Epstein-Barr virus (CAEBV) infection is a systemic Epstein-Barr virus (EBV)-positive lymphoproliferative disorder characterized by persistent or recurrent infectious mononucleosis-like symptoms in patients with no known immunodeficiency. The detailed pathogenesis of the disease is unknown and no standard treatment regimen has been developed. EBV gene expression was analysed in peripheral blood samples collected from 24 patients with CAEBV infection. The expression levels of six latent and two lytic EBV genes were quantified by real-time RT-PCR. EBV-encoded small RNA 1 and BamHI-A rightward transcripts were abundantly detected in all patients, and latent membrane protein (LMP) 2 was observed in most patients. EBV nuclear antigen (EBNA) 1 and LMP1 were detected less frequently and were expressed at lower levels. EBNA2 and the two lytic genes were not detected in any of the patients. The pattern of latent gene expression was determined to be latency type II. EBNA1 was detected more frequently and at higher levels in the clinically active patients. Quantifying EBV gene expression is useful in clarifying the pathogenesis of CAEBV infection and may provide information regarding a patient's disease prognosis, as well as possible therapeutic interventions.
慢性活动性 EBV 病毒（CAEBV）感染是一种以 EBV 阳性的全身性淋巴组织增生性疾病为特征的疾病，其特点是患者无已知免疫缺陷，但持续或反复出现类似于传染性单核细胞增多症的症状。该疾病的详细发病机制尚不清楚，也尚未制定出标准的治疗方案。本研究分析了 24 例 CAEBV 感染患者外周血样本中的 EBV 基因表达。通过实时 RT-PCR 定量检测了 6 种潜伏和 2 种裂解 EBV 基因的表达水平。所有患者均大量检测到 EBV 编码的小 RNA 1 和 BamHI-A 右向转录物，大多数患者观察到潜伏膜蛋白（LMP）2。EBV 核抗原（EBNA）1 和 LMP1 的检出频率较低，表达水平也较低。在任何患者中均未检测到 EBNA2 和两种裂解基因。潜伏基因表达模式被确定为潜伏类型 II。在临床上活跃的患者中，EBNA1 的检出频率更高，表达水平也更高。定量检测 EBV 基因表达有助于阐明 CAEBV 感染的发病机制，并可能为患者的疾病预后以及可能的治疗干预措施提供信息。
