McWhirter R B, Klapper M H
Department of Chemistry, Ohio State University, Columbus 43210.
Biochemistry. 1990 Jul 24;29(29):6919-26. doi: 10.1021/bi00481a023.
Methoxatin is a novel o-quinone coenzyme found in a variety of dehydrogenases and oxidases. In this paper we present the visible absorption spectra, apparent pKa, and midpoint potentials of the methoxatin and two analogous phenanthrolinedione semiquinones. We have also obtained absorption spectra for the semiquinone forms of the methoxatin-containing holoenzyme methylamine dehydrogenase and of its resolved methoxatin-containing subunit. The two protein and the methoxatin semiquinone spectra all differ from one another.