带有带电光可裂解质量标记的肽核酸探针：迈向基于肽核酸的基质辅助激光解吸电离飞行时间质谱基因分析
Peptide nucleic acid probes with charged photocleavable mass markers: Towards PNA-based MALDI-TOF MS genetic analysis.
作者信息
Ball Rachel J, Green Philip S, Gale Nittaya, Langley G John, Brown Tom
机构信息
School of Chemistry; University of Southampton; Southampton, UK.
出版信息
Artif DNA PNA XNA. 2010 Jul;1(1):27-35. doi: 10.4161/adna.1.1.12199.
Abstract
Halogen-labelled peptide organic acid (HPOA) monomers have been synthesised and incorporated into sequence-specific peptide nucleic acid (PNA) probes. Three different types of probe have been prepared; the unmodified PNA probe, the PNA probe with a mass marker, and the PNA probe with photocleavable mass marker. All three types of probe have been used in model studies to develop a mass spectrometry-based hybridisation assay for detection of point mutations in DNA.
摘要
已合成卤代标记的肽类有机酸（HPOA）单体，并将其掺入序列特异性肽核酸（PNA）探针中。制备了三种不同类型的探针；未修饰的PNA探针、带有质量标记物的PNA探针和带有光可裂解质量标记物的PNA探针。所有这三种类型的探针都已用于模型研究，以开发一种基于质谱的杂交分析方法来检测DNA中的点突变。
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