Gale Nittaya, Kocalka Petr, Mardle Charlotte, Brown Tom
School of Chemistry, University of Southampton, Southampton, UK.
Artif DNA PNA XNA. 2011 Jul-Dec;2(3):79-89. doi: 10.4161/adna.18179.
Aminoalkyl and alkyne-tagged PNA HyBeacons have been synthesized, labeled with fluorescein via conventional amide bond or triazole formation (click chemistry) and used to detect single nucleotide polymorphisms (SNPs) implicated in statin-induced myopathy. The PNA HyBeacons gave much better mismatch/mutant discrimination than conventional DNA HyBeacons but smaller fluorescence changes on melting.
已合成了带有氨基烷基和炔烃标签的肽核酸（PNA）杂交信标，通过常规酰胺键或三唑形成（点击化学）用荧光素标记，并用于检测与他汀类药物引起的肌病相关的单核苷酸多态性（SNP）。与传统的DNA杂交信标相比，PNA杂交信标在错配/突变鉴别方面表现得更好，但在解链时荧光变化较小。
