简便制备可回收的活性纳米生物催化剂：一锅法用 Ni-NTA 功能化磁性纳米粒子纯化和固定化酶。
Facile fabrication of recyclable and active nanobiocatalyst: purification and immobilization of enzyme in one pot with Ni-NTA functionalized magnetic nanoparticle.
机构信息
Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore 117576.
出版信息
Chem Commun (Camb). 2011 Jul 28;47(28):8115-7. doi: 10.1039/c1cc12685g. Epub 2011 Jun 20.
PMID:21687876
Abstract
Ni-NTA functionalized iron oxide magnetic nanoparticle was synthesized and used to selectively immobilize a his-tagged enzyme from cell free extract as an active and recyclable nanobiocatalyst, where purification and immobilization of the target enzyme were accomplished in one pot.
摘要
镍-氮三乙酸功能化氧化铁磁性纳米粒子被合成，并用于从无细胞提取物中选择性固定一个组氨酸标记的酶作为一种活性和可回收的纳米生物催化剂，其中目标酶的纯化和固定化在一个步骤中完成。
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