Institute of Ecology and Genetics of Microorganisms, Russian Academy of Sciences, ul.Goleva 13, Perm 614081, Russia.
Microbiol Res. 2012 Mar 20;167(3):166-72. doi: 10.1016/j.micres.2011.05.005.
Glutathione (GSH) plays an important role in bacterial cells, participating in maintenance of redox balance in the cytoplasm and in defense against many toxic compounds and stresses. In this study we demonstrate that in aerobic, exponentially growing Escherichia coli culture endogenous reduced glutathione undergoes continuous transmembrane cycling between the cells and medium. As a result of an establishment of a dynamic balance between GSH efflux and uptake, a constant extracellular concentration of GSH counting per biomass unit is maintained. The magnitude of this concentration strictly depends on external pH. GSH cycling is carried out in respiring cells and disturbed by influences, which change the level of ΔμH(+) and ATP. Export of GSH is modified by phosphate deficiency in the medium.
谷胱甘肽（GSH）在细菌细胞中起着重要作用，参与细胞质中氧化还原平衡的维持以及许多有毒化合物和应激的防御。在本研究中，我们证明在有氧、指数生长的大肠杆菌培养物中，内源性还原型谷胱甘肽在细胞和培养基之间进行连续的跨膜循环。由于 GSH 外排和摄取之间建立了动态平衡，因此每生物质单位计数的细胞外 GSH 浓度保持恒定。该浓度的大小严格取决于外部 pH 值。GSH 循环在呼吸细胞中进行，并受到影响，这些影响会改变 ΔμH(+)和 ATP 的水平。GSH 的外排受培养基中磷酸盐缺乏的影响。
