一种 Ru(II) 配合物与 2-(4-(甲磺酰基)苯基)-1H-咪唑[4,5-f][1,10]菲咯啉的合成、表征及酸碱和 DNA 结合性质。
A Ru(II) complex with 2-(4-(methylsulfonyl)phenyl)-1H-imidazo[4,5-f][1,10]phenanthroline: synthesis, characterization, and acid-base and DNA-binding properties.
机构信息
College of Chemistry, Beijing Normal University, Beijing 100875, China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2011 Sep;79(5):1815-22. doi: 10.1016/j.saa.2011.05.063. Epub 2011 May 31.
A new Ru(II) complex of [Ru(bpy)2(Hmspip)]Cl2 {in which bpy=2,2'-bipyridine, Hmspip=2-(4-(methylsulfonyl)phenyl)-1H-imidazo[4,5-f][1,10]phenanthroline} have been synthesized and characterized. The ground- and excited-state acid-base properties of [Ru(bpy)2(Hmspip)]Cl2 and its parent complex of [Ru(bpy)2(Hpip)]Cl2 {Hpip=2-phenyl-1H-imidazo[4,5-f][1,10]phenanthroline} have been studied by UV-visible (UV-vis) and emission spectrophotometric pH titrations. [Ru(bpy)2(Hmspip)]Cl2 acts as a calf thymus DNA intercalators with a binding constant of 4.0×10(5) M(-1) in buffered 50 mM NaCl, as evidenced by UV-vis and luminescence titrations, steady-state emission quenching by [Fe(CN)6]4-, DNA competitive binding with ethidium bromide, reverse salt titrations and viscosity measurements.
一种新的 Ru(II) 配合物 [Ru(bpy)2(Hmspip)]Cl2 已经被合成并进行了表征，其中 bpy=2,2'-联吡啶，Hmspip=2-(4-(甲基磺酰基)苯基)-1H-咪唑[4,5-f][1,10]菲咯啉。通过紫外-可见（UV-vis）和荧光分光光度 pH 滴定法研究了 [Ru(bpy)2(Hmspip)]Cl2 及其母体配合物 [Ru(bpy)2(Hpip)]Cl2（Hpip=2-苯基-1H-咪唑[4,5-f][1,10]菲咯啉）的基态和激发态酸碱性质。[Ru(bpy)2(Hmspip)]Cl2 作为小牛胸腺 DNA 嵌入剂，在缓冲的 50mM NaCl 中具有 4.0×10(5) M(-1)的结合常数，这可以通过紫外-可见和荧光滴定、[Fe(CN)6]4-的稳态荧光猝灭、与溴化乙锭的 DNA 竞争结合、反盐滴定和粘度测量来证明。
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