两个咔唑基团的线性组装对钌（II）配合物的酸碱和 DNA 结合性质的影响。
The effects of linear assembly of two carbazole groups on acid-base and DNA-binding properties of a ruthenium(II) complex.
机构信息
College of Chemistry and Beijing Key Laboratory of Energy Conversion and Storage Materials, Beijing Normal University, Beijing 100875, China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2013 Jul;111:196-203. doi: 10.1016/j.saa.2013.04.017. Epub 2013 Apr 10.
A novel Ru(II) complex of Ru(bpy)2(Hbcpip)2 {where bpy=2,2-bipyridine, Hbcpip=2-(4-(9H-3,9'-bicarbazol-9-yl)phenyl)-1H-imidazo[4,5-f][1,10]phenanthroline} is synthesized and characterized. Calf-thymus DNA-binding properties of the complex were studied by UV-vis absorption and luminescence titrations, steady-state emission quenching by Fe(CN)6, DNA competitive binding with ethidium bromide, thermal denaturation and DNA viscosity measurements. The results indicate that the complex partially intercalated into the DNA with a binding constant of (5.5±1.4)×10(5) M(-1) in buffered 50 mM NaCl. The acid-base properties of the complex were also studied by UV-visible and luminescence spectrophotometric pH titrations, and ground- and excited-state acidity ionization constant values were derived.
合成并表征了一种新型的 Ru(bpy)2(Hbcpip)2（其中 bpy=2,2-联吡啶，Hbcpip=2-(4-(9H-3,9'-联咔唑-9-基)苯基)-1H-咪唑[4,5-f][1,10]菲咯啉）的 Ru(II) 配合物。通过紫外可见吸收和荧光滴定、Fe(CN)6的稳态发射猝灭、与溴化乙锭的 DNA 竞争性结合、热变性和 DNA 粘度测量研究了配合物与小牛胸腺 DNA 的结合性质。结果表明，该配合物部分插入 DNA 中，在缓冲的 50mM NaCl 中结合常数为(5.5±1.4)×10(5)M(-1)。还通过紫外可见和荧光分光光度 pH 滴定研究了配合物的酸碱性质，并得出了基态和激发态酸度电离常数值。
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