Saibara T, Onishi S, Matsuura Y, Fujikawa M, Sakaeda H, Matsunaga Y, Yamamoto Y
First Department of Medicine, Kochi Medical School, Japan.
Biotherapy. 1990;2(1):1-12. doi: 10.1007/BF02172071.
Hepatocellular carcinoma (HCC) patients can be divided into two groups according to the degree of lymphokine activated killer (LAK) cell activity; a high LAK activity group (H-LAK-HCC) and a low LAK activity group (L-LAK-HCC). Interferon-gamma (IFN-gamma) production is severely defective in L-LAK-HCC but not defective in H-LAH-HCC. IFN-gamma production is suppressed with the addition of anti-Tac in dose dependent manner, though LAK activity is suppressed only in the presence of high concentration of anti-Tac. LAK activity is suppressed with the addition of anti-IFN-gamma, which is most prominent when the antibody is present during the first 12 hr of incubation. LAK generation is enhanced with the addition of recombinant IFN-gamma, which is most prominent when it is present during the first 12 hr of incubation. However, this enhancing effect is less prominent in L-LAK-HCC as compared to normals, liver cirrhosis, and H-LAK-HCC. This enhancement is regarded to depend on the presence of Leu7+ and Leu11+ subset, as this enhancement is abandoned and IFN-gamma production is inhibited when either of these subsets is deleted. These data suggest that IFN-gamma production and the participation of Leu7+ and Leu11+ subsets is important in sufficient LAK generation, and that poor IFN-gamma production and insufficient response to IFN-gamma may be the cause of severely defective LAK generation in L-LAK-HCC.
肝细胞癌（HCC）患者可根据淋巴因子激活的杀伤细胞（LAK）活性程度分为两组；高LAK活性组（H-LAK-HCC）和低LAK活性组（L-LAK-HCC）。γ干扰素（IFN-γ）的产生在L-LAK-HCC中严重缺陷，但在H-LAH-HCC中无缺陷。添加抗Tac后，IFN-γ的产生以剂量依赖方式受到抑制，尽管只有在高浓度抗Tac存在时LAK活性才受到抑制。添加抗IFN-γ后LAK活性受到抑制，当抗体在孵育的前12小时存在时这种抑制最为明显。添加重组IFN-γ可增强LAK的产生，当它在孵育的前12小时存在时这种增强最为明显。然而，与正常、肝硬化和H-LAK-HCC相比，这种增强作用在L-LAK-HCC中不太明显。这种增强被认为取决于Leu7+和Leu11+亚群的存在，因为当这些亚群中的任何一个被删除时，这种增强作用就会消失且IFN-γ的产生会受到抑制。这些数据表明，IFN-γ的产生以及Leu7+和Leu11+亚群的参与对于充分产生LAK很重要，并且IFN-γ产生不足和对IFN-γ反应不足可能是L-LAK-HCC中LAK产生严重缺陷的原因。
