Center for Infectious Disease Research, Instituto Nacional de Salud Publica, Cuernavaca, Morelos, Mexico.
Acta Trop. 2011 Oct-Nov;120(1-2):67-71. doi: 10.1016/j.actatropica.2011.06.002. Epub 2011 Jun 12.
Increased levels of tumor necrosis factor alpha (TNF-α) in patients with dengue have been reported. Various polymorphisms have been identified in the promoter region of the TNF-α gene that may affect its transcription. The purpose of the present study was to evaluate the relationship between polymorphisms of TNF-α gene and the genetic susceptibility to dengue fever in a group of patients from Morelos State, Mexico. The TNF-α polymorphisms (positions -238 and -308) were determined by PCR-RFLP technique in 130 patients with dengue (85 with dengue fever and 45 with dengue hemorrhagic fever) and 169 healthy controls. The patients were selected from cases reported in Morelos State from 1997 to 2003. The whole group of dengue patients showed a decreased frequency of TNF-α -238 A allele when compared to healthy controls (p = 0.01, OR = 0.19, 95%CI = 0.02-0.78). When the analysis was made separately in dengue fever and dengue hemorrhagic fever patients, the decreased frequency of TNF-α -238 A allele only remained significant in patients with DHF when compared to healthy controls (p = 0.034). This work suggests a possible association of TNF-α -238 A allele with protection to develop symptomatic disease.
已有报道称登革热患者体内肿瘤坏死因子-α（TNF-α）水平升高。在 TNF-α 基因启动子区域已经发现了多种可能影响其转录的多态性。本研究的目的是评估在墨西哥莫雷洛斯州的一组患者中，TNF-α 基因多态性与登革热易感性的关系。采用 PCR-RFLP 技术检测 130 例登革热患者（85 例登革热和 45 例登革出血热）和 169 例健康对照者 TNF-α 多态性（-238 和 -308 位）。这些患者是从 1997 年至 2003 年莫雷洛斯州报告的病例中选择的。与健康对照组相比，所有登革热患者 TNF-α-238A 等位基因的频率均降低（p = 0.01，OR = 0.19，95%CI = 0.02-0.78）。当在登革热和登革出血热患者中分别进行分析时，只有 DHF 患者 TNF-α-238A 等位基因的频率与健康对照组相比仍显著降低（p = 0.034）。本研究提示 TNF-α-238A 等位基因可能与出现症状性疾病的保护作用有关。
