Suda T, Sumitomo T, Nakano Y, Tozawa F, Ushiyama T, Demura H
Department of Medicine, Tokyo Women's Medical College, Japan.
J Clin Endocrinol Metab. 1990 Oct;71(4):913-7. doi: 10.1210/jcem-71-4-913.
The binding of CRH-binding protein (CRH-BP) in plasma to labeled human CRH has been examined in patients with hypothalamic-pituitary-adrenal disorders. Compared with that in normal subjects, CRH-BP binding decreased in patients with Cushing's syndrome of pituitary or adrenal origin and in patients who were treated with a high dose of glucocorticoids over a long period of time. On the other hand, CRH-BP binding increased in patients with Addison's disease or hypopituitarism. In patients with Addison's disease, the high level of CRH-BP binding fell to the control level after glucocorticoid replacement. In patients with Cushing's syndrome, CRH-BP binding gradually increased and reached the higher level about 1 yr after surgery. Thereafter, it returned to the control level. There was a good negative correlation between the levels of plasma cortisol and CRH-BP binding in patients with Cushing's syndrome before and after surgery. A Scatchard analysis of CRH-BP binding in patients with Cushing's syndrome and in normal subjects showed that the binding affinity was similar in both groups, but that the number of binding sites was low in patients with Cushing's syndrome. These results suggest that in human plasma, glucocorticoids decrease CRH-BP binding. This seems to be caused by a decrease in the concentration of CRH-BP in the plasma of patients with hypercortisolemia.
在患有下丘脑 - 垂体 - 肾上腺疾病的患者中，已对血浆中促肾上腺皮质激素释放激素结合蛋白（CRH - BP）与标记的人促肾上腺皮质激素释放激素（CRH）的结合情况进行了研究。与正常受试者相比，垂体或肾上腺源性库欣综合征患者以及长期接受高剂量糖皮质激素治疗的患者中，CRH - BP结合减少。另一方面，艾迪生病或垂体功能减退患者的CRH - BP结合增加。在艾迪生病患者中，糖皮质激素替代治疗后，高水平的CRH - BP结合降至对照水平。在库欣综合征患者中，CRH - BP结合逐渐增加，并在手术后约1年达到较高水平。此后，它恢复到对照水平。库欣综合征患者手术前后血浆皮质醇水平与CRH - BP结合水平之间存在良好的负相关。对库欣综合征患者和正常受试者的CRH - BP结合进行Scatchard分析表明，两组的结合亲和力相似，但库欣综合征患者的结合位点数较低。这些结果表明，在人血浆中，糖皮质激素会降低CRH - BP结合。这似乎是由于高皮质醇血症患者血浆中CRH - BP浓度降低所致。
