Ohmori N, Suda T, Sato Y, Kasagi Y, Tozawa H, Dobashi I, Demura H
Department of Medicine, Tokyo Women's Medical College, Japan.
Endocr J. 1994 Oct;41(5):553-8. doi: 10.1507/endocrj.41.553.
Immunoreactive corticotropin-releasing factor-binding protein (CRF-BP) concentrations in human plasma were determined by means of radioimmunoassay for human CRF-BP. CRF-BP antiserum to the C-terminal fragment of human CRF-BP (298-322) was produced, and CRF-BP (298-322) was used as the tracer and the standard. Large amounts of human CRF did not affect measurement of plasma CRF-BP with this radioimmunoassay. The basal plasma CRF-BP concentration in normal subjects was 4.19 +/- 0.57 nmol/L (mean +/- SD). The CRF-BP concentration was low in patients with Cushing's syndrome, except those with preclinical Cushing's syndrome, and high in patients with Addison's disease, hypopituitarism and isolated ACTH deficiency. After surgery, the plasma CRF-BP concentration in patients with Cushing's syndrome rose, peaked, and then decreased to the control level. In patients with Addison's disease, the high plasma CRF-BP concentration decreased to the control level after hydrocortisone replacement, the same as plasma ACTH concentration. These findings suggest that the immunoreactive CRF-BP concentration in human plasma was decreased by glucocorticoids, at least under chronic conditions.
采用人促肾上腺皮质激素释放因子结合蛋白（CRF - BP）放射免疫分析法测定人血浆中免疫反应性CRF - BP的浓度。制备了针对人CRF - BP C末端片段（298 - 322）的CRF - BP抗血清，并将CRF - BP（298 - 322）用作示踪剂和标准品。大量的人促肾上腺皮质激素释放因子（CRF）并不影响该放射免疫分析法对血浆CRF - BP的测定。正常受试者的基础血浆CRF - BP浓度为4.19±0.57 nmol/L（平均值±标准差）。库欣综合征患者（除临床前期库欣综合征患者外）的CRF - BP浓度较低，而艾迪生病、垂体功能减退症和孤立性促肾上腺皮质激素（ACTH）缺乏症患者的CRF - BP浓度较高。手术后，库欣综合征患者的血浆CRF - BP浓度升高、达到峰值，然后降至对照水平。在艾迪生病患者中，氢化可的松替代治疗后，血浆CRF - BP的高浓度降至对照水平，与血浆ACTH浓度情况相同。这些发现表明，至少在慢性条件下，糖皮质激素可降低人血浆中免疫反应性CRF - BP的浓度。
