Nagano S, Umemoto M, Kobayashi K, Matsuoka A
Department of Clinical Pathology, Hyogo College of Medicine, Nishinomiya.
Rinsho Byori. 1990 Jul;38(7):794-8.
Nitroblue tetrazolium (NBT) is reduced to a blue dye (blue formazan, gamma max 560 nm) by superoxide (O2-.) released from phagocytes upon phorbol myristate acetate-stimulation, resulting in the blue dye-deposition on the cell membrane. Thus, we tried to develop a semi-quantification method for determining the NBT-reducing activity in the peripheral leukocytes (NBT-staining intensity -SI- and its distribution in the cell) by flow cytometry. The SI (mean +/- SD) was 28.4 +/- 2.2 (n = 30) in normal subjects and 0.9 in a patient with chronic granulomatous disease, whereas it was 37.6 in a patient with malaria. In addition, patients with myelodysplastic syndrome showed values of 29.2 +/- 7.0 (n = 11), while a patient with partial monosomy of chromosome No. 7 had an SI of 10.8. The present assay of NBT-reducing activity in phagocytes may be useful for screening of peripheral leukocyte function.
在佛波醇肉豆蔻酸酯乙酸盐刺激下，吞噬细胞释放的超氧化物（O2-·）可将硝基蓝四氮唑（NBT）还原为蓝色染料（蓝色甲臜，最大吸收波长560nm），导致蓝色染料沉积在细胞膜上。因此，我们试图开发一种半定量方法，通过流式细胞术测定外周血白细胞中的NBT还原活性（NBT染色强度-SI-及其在细胞中的分布）。正常受试者的SI（平均值±标准差）为28.4±2.2（n = 30），慢性肉芽肿病患者为0.9，而疟疾患者为37.6。此外，骨髓增生异常综合征患者的值为29.2±7.0（n = 11），而7号染色体部分单体患者的SI为10.8。目前对吞噬细胞中NBT还原活性的检测可能有助于外周血白细胞功能的筛查。
