Bröker M, Abel K J, Köhler R, Hilfenhaus J, Amann E
Research Laboratories of Behringwerke AG, Marburg, Federal Republic of Germany.
Med Microbiol Immunol. 1990;179(3):145-59. doi: 10.1007/BF00202392.
Immunization studies with HSV-1 and HSV-2 envelope proteins expressed in Escherichia coli were performed. After active immunization of mice with a gD-1 antigen (Leu53-Ala312) expressed as a fusion protein, the animals were protected from a lethal challenge with HSV-1 and HSV-2. In addition, antisera from rabbits immunized with the same gD-1 antigen also conferred passive immunity to mice against a challenge infection with either HSV-1 or HSV-2. In contrast to these successful gD-1 protection experiments, various gC-1 and gC-2 fusion proteins from E. coli failed to induce protective immunity. Moreover, the mice sera from immunized animals were not able to react with the authentic, glycosylated gC-1 and gC-2 envelope proteins, whereas sera raised against authentic gC-1 and gC-2 glycoproteins do recognize the gC fusion proteins from E. coli. These results indicate, that E. coli might represent an ideal system for expressing gD antigens as a possible component of a HSV vaccine, whereas gC antigen cannot be produced in an immunocompetent form in E. coli.
开展了对在大肠杆菌中表达的单纯疱疹病毒1型（HSV-1）和单纯疱疹病毒2型（HSV-2）包膜蛋白的免疫研究。在用作为融合蛋白表达的gD-1抗原（Leu53-Ala312）对小鼠进行主动免疫后，这些动物受到HSV-1和HSV-2致死性攻击时得到了保护。此外，用相同gD-1抗原免疫的兔的抗血清也赋予小鼠针对HSV-1或HSV-2攻击感染的被动免疫。与这些成功的gD-1保护实验相反，来自大肠杆菌的各种gC-1和gC-2融合蛋白未能诱导保护性免疫。此外，免疫动物的小鼠血清不能与天然的、糖基化的gC-1和gC-2包膜蛋白发生反应，而针对天然gC-1和gC-2糖蛋白产生的血清确实能识别来自大肠杆菌的gC融合蛋白。这些结果表明，大肠杆菌可能是表达gD抗原作为HSV疫苗可能成分的理想系统，而gC抗原不能在大肠杆菌中以具有免疫活性的形式产生。
