cDNA克隆的轻松鉴定。
Easy identification of cDNA clones.
作者信息
Rüther U, Müller-Hill B
出版信息
EMBO J. 1983;2(10):1791-4. doi: 10.1002/j.1460-2075.1983.tb01659.x.
Abstract
A set of six cloning vectors, pUR 278, 288, 289, 290, 291, 292 is presented. These vectors have the cloning sites, BamHI, SalI, PstI, XbaI and HindIII, in all frames at the 3' end of the lacZ gene. Insertion of cDNA in the proper cloning sites leads to a fusion protein of active beta-galactosidase and the peptide encoded by the cDNA. A simple immunoenzymatic assay can be used to identify clones in such a cDNA library.
摘要
本文介绍了一组六个克隆载体，即pUR 278、288、289、290、291、292。这些载体在lacZ基因3'端的所有读框中都有克隆位点BamHI、SalI、PstI、XbaI和HindIII。将cDNA插入适当的克隆位点会产生活性β-半乳糖苷酶与cDNA编码的肽的融合蛋白。一种简单的免疫酶测定法可用于在此类cDNA文库中鉴定克隆。
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