Mellanby J, Matsuda M, Sugimoto N, Lei D L, Bizzini B
University of Oxford, Department of Experimental Psychology, Oxford.
Toxicon. 1990;28(6):737-41. doi: 10.1016/0041-0101(90)90266-a.
While 4 micrograms of Fragment A-B of tetanus toxin (which lacks the binding site for nervous tissue) causes flaccid paralysis and death in mice, 26 micrograms has no toxic effect in goldfish. Antibodies to either A-B or to fragment C (which contains the binding site) block the paralytic effect of whole toxin in goldfish. It is concluded that binding is necessary for the neuromuscular blocking action of the toxin in goldfish.
虽然4微克破伤风毒素的A - B片段（缺乏与神经组织的结合位点）可导致小鼠出现弛缓性麻痹和死亡，但26微克该片段对金鱼却没有毒性作用。针对A - B片段或C片段（含有结合位点）的抗体可阻断全毒素对金鱼的麻痹作用。由此得出结论，结合对于毒素在金鱼体内的神经肌肉阻断作用是必要的。
