Department of Engineering Sciences, Middle East Technical University, Ankara, Turkey.
J Biosci Bioeng. 2011 Sep;112(3):279-88. doi: 10.1016/j.jbiosc.2011.05.014. Epub 2011 Jun 22.
Sericin, a silk protein, has high potential for use in biomedical applications. In this study, wound dressing membranes of Sericin (S) and Collagen (C) were prepared by glutaraldehyde cross-linking at S/C; 2:1, 1:1, 1:2, and 0:1 weight ratios. They were stable in water for 4 weeks. However, increasing the proportion of sericin had decreasing effect on the membrane stability. Water swelling property of membranes was enhanced with sericin. The highest water swelling was obtained in 1:1 group (9.06 g/g), but increasing collagen or sericin content in the membranes had a diminishing effect. Highest water vapor transmission rate was obtained with 1:2 group (1013.80 g/m(2)/day). Oxygen permeability results showed that 1:2 (7.67 mg/L) and 2:1 (7.85 mg/L) S/C groups were better than the other groups. While sericin decreased the tensile strength and elongation of membranes, it increased modulus. Sericin also increased brittleness of membranes, but their UTS range (24.93-44.92 MPa) was still suitable for a wound dressing. Membranes were not penetrable to microorganisms. Cytotoxicity studies showed that fibroblasts and keratinocytes attached and gained their characteristic morphologies. They also proliferated on membranes significantly. After 1 week of subcutaneous implantation, a fibrous capsule formed around all membranes with an acute inflammation. Sericin containing membranes showed signs of degradation (at 2nd week), while collagen only membranes remained largely intact. Eventually, sericin containing membranes degraded in 3 weeks with moderate inflammatory response. Overall results suggest that sericin/collagen membranes would be favorable as wound dressing material when sericin ratio is less than or equal to the collagen component.
丝胶是一种丝蛋白，在生物医学应用中具有很高的应用潜力。在这项研究中，通过戊二醛交联制备了丝胶（S）和胶原蛋白（C）的伤口敷料膜，S/C 为 2:1、1:1、1:2 和 0:1 重量比。它们在水中稳定 4 周。然而，增加丝胶的比例会降低膜的稳定性。膜的水膨胀性能随丝胶的增加而增强。在 1:1 组中获得了最高的水膨胀率（9.06 g/g），但在膜中增加胶原蛋白或丝胶含量会产生递减效应。1:2 组（1013.80 g/m²/天）获得了最高的水蒸气透过率。氧气渗透率结果表明，1:2（7.67 mg/L）和 2:1（7.85 mg/L）的 S/C 组优于其他组。虽然丝胶降低了膜的拉伸强度和伸长率，但增加了模量。丝胶还增加了膜的脆性，但它们的 UTS 范围（24.93-44.92 MPa）仍适用于伤口敷料。膜对微生物不可渗透。细胞毒性研究表明，成纤维细胞和角质形成细胞附着并获得其特征形态。它们在膜上也显著增殖。皮下植入 1 周后，所有膜周围都形成了一个纤维囊，伴有急性炎症。含丝胶的膜显示出降解的迹象（第 2 周），而仅含胶原蛋白的膜基本保持完整。最终，含丝胶的膜在 3 周内降解，伴有中度炎症反应。总体结果表明，当丝胶比例小于或等于胶原蛋白成分时，丝胶/胶原蛋白膜将作为伤口敷料材料是有利的。
