O'Brien T W, Denslow N D, Anders J C, Courtney B C
Department of Biochemistry and Molecular Biology, University of Florida, Gainesville.
Biochim Biophys Acta. 1990 Aug 27;1050(1-3):174-8. doi: 10.1016/0167-4781(90)90162-u.
Oligoribonucleotides and mRNA were used to define properties of the bovine mitoribosomal mRNA binding site. The RNA binding domain on the 28 S subunit spans approx. 80 nucleotides of the template, based on ribosome protection experiments, but the major interaction with the ribosome occurs over a 30 nucleotide stretch. The binding site for E. coli IF3 is conserved in bovine mitoribosomes, but mitochondrial factors appear essential for proper interaction of mRNA with mitoribosomes. The small subunit of bovine mitoribosomes contains a high-affinity binding site for guanyl nucleotides, further indication of specialized mechanisms for initiation complex formation and function of mammalian mitochondrial ribosomes.
寡核糖核苷酸和信使核糖核酸被用于确定牛线粒体核糖体信使核糖核酸结合位点的特性。基于核糖体保护实验，28 S亚基上的RNA结合结构域跨越模板上约80个核苷酸，但与核糖体的主要相互作用发生在30个核苷酸的区域内。大肠杆菌IF3的结合位点在牛线粒体核糖体中保守，但线粒体因子似乎对信使核糖核酸与线粒体核糖体的正确相互作用至关重要。牛线粒体核糖体的小亚基含有一个对鸟苷核苷酸具有高亲和力的结合位点，这进一步表明了哺乳动物线粒体核糖体起始复合物形成和功能的特殊机制。
