[体外生理条件下及体内组织中抗坏血酸与铁络合物的形成]

[Formation of iron complexes with ascorbic acid in physiological conditions in vitro and in tissue in vivo].

作者信息

Kozlov A V, Egorov D Iu, Vladimirov Iu A, Azizova O A

出版信息

Biofizika. 1990 May-Jun;35(3):513-7.

Abstract

Rapid dissociated Fe(III)-ascorbic acid complexes are formed after the mixing of Fe(II) (100 mkM) with ascorbic acid (1 mM) at pH-7,4. As a result of these complexes formation Fe(II) concentration was decreased in solution, but o-phenantrolin addition or pH decreased up to 6.1 lead to the destruction of these complexes and reduction of iron up to Fe(II). Iron-ascorbic acid paramagnetic complexes were not observed in the perfused rat liver homogenate. Probably, these facts were due to the presence of other stronger reductants in the tissue.

摘要

在pH值为7.4的条件下，将100 μM的Fe(II)与1 mM的抗坏血酸混合后，会迅速形成离解的Fe(III)-抗坏血酸复合物。这些复合物形成后，溶液中的Fe(II)浓度降低，但加入邻菲罗啉或将pH值降至6.1会导致这些复合物的破坏以及铁还原为Fe(II)。在灌注的大鼠肝脏匀浆中未观察到铁-抗坏血酸顺磁性复合物。这些事实可能是由于组织中存在其他更强的还原剂。