Yang Yanjing, Yan Huidan, Li Yuqin, Yang Shang-Tian, Zhang Xuewu
College of Light Industry and Food Sciences, South China University of Technology, Guangzhou China.
Pharmazie. 2011 May;66(5):382-90.
Isothiocyanates (ITCs) have been shown to possess antitumor activity in colon cancer, however, the detailed mechanism is still unclear. The objective of this study was to investigate apoptosis-inducing activity of ITCs from Broccolini seeds and proteomic changes in SW480 cells, and to identify the molecular pathways responsible for the anticancer action of ITCs. We found that ITCs induces SW480 cells apoptosis in a dose-dependent manner by using MTT assay, phase contrast microscope and flow cytometry, and the IC50 was calculated to be 77.72 microg/ml, superior to the chemotherapeutical drug 5-flurouracil. Subsequently, 15 altered proteins in ITCs treated SW480 cells were identified. Further bioinformatics analysis predicted the potential pathways for ITCs to induce apoptosis of SW480 cells. In conclusion, this is the first report to investigate anticancer activity of ITCs from Broccolini seeds and its mechanism of action by proteomics analysis. Our observations provide potential therapeutic targets for colon cancer inhibitor intervention and implicate the development of novel anti-cancer therapeutic strategies.
异硫氰酸盐（ITCs）已被证明在结肠癌中具有抗肿瘤活性，然而，其详细机制仍不清楚。本研究的目的是研究西兰花种子中的ITCs诱导SW480细胞凋亡的活性以及蛋白质组学变化，并确定负责ITCs抗癌作用的分子途径。我们通过MTT法、相差显微镜和流式细胞术发现ITCs以剂量依赖的方式诱导SW480细胞凋亡，计算得出IC50为77.72微克/毫升，优于化疗药物5-氟尿嘧啶。随后，鉴定出ITCs处理的SW480细胞中15种改变的蛋白质。进一步的生物信息学分析预测了ITCs诱导SW480细胞凋亡的潜在途径。总之，这是第一份通过蛋白质组学分析研究西兰花种子中ITCs抗癌活性及其作用机制的报告。我们的观察结果为结肠癌抑制剂干预提供了潜在的治疗靶点，并暗示了新型抗癌治疗策略的发展。
