Department of Comprehensive Cancer Care, Masaryk Memorial Cancer Institute, Brno, Czech Republic.
Eur J Med Res. 2012 Jun 14;17(1):17. doi: 10.1186/2047-783X-17-17.
Central Europe presents with the highest incidence of sporadic colorectal cancer (CRC) worldwide. As sporadic CRC represents a typical multifactorial disease, it is characterized by intense interaction of the genetic background with the environment. Glutathione S-transferases could act as attractive susceptibility genes for CRC, as they are directly involved in conjugation between glutathione and chemotherapeutics, environmental pollutants and a wide spectrum of xenobiotics.
In this study, we investigated associations of polymorphisms in glutathione S-transferases (GSTs) genes, that is GSTA1, GSTT1, GSTM1 and GSTP1, with CRC in a total of 197 cases and 218 controls originating from the Czech Central European population. Polymorphisms were assessed by polymerase chain reaction/restriction fragment length polymorphism-based methods, allele-specific multiplex and allelic discrimination by real-time polymerase chain reaction.
None of investigated polymorphisms showed any associations with CRC, with the exception of GSTP1; where the heterozygote genotype Ile105Val was associated with decreased risk of CRC (P = 0.043).
The frequencies observed in our study are in accordance with those from other European Caucasian populations. Based on our studies, examined variability in GST genes is not a major determinant of CRC susceptibility in the Central European population.
中欧地区的散发性结直肠癌（CRC）发病率居世界首位。由于散发性 CRC 是一种典型的多因素疾病，其特征是遗传背景与环境之间的强烈相互作用。谷胱甘肽 S-转移酶（GSTs）可能作为 CRC 的易感基因，因为它们直接参与谷胱甘肽与化疗药物、环境污染物和广泛的外源性物质之间的结合。
本研究共纳入 197 例 CRC 患者和 218 例来自捷克中欧人群的对照，采用聚合酶链反应/限制性片段长度多态性（PCR/RFLP）、等位基因特异性多重 PCR 和实时 PCR 等位基因鉴别方法，检测 GSTs 基因（GSTA1、GSTT1、GSTM1 和 GSTP1）的多态性。
除 GSTP1 外，未发现其他任何多态性与 CRC 相关，其中 GSTP1 的 Ile105Val 杂合基因型与 CRC 风险降低相关（P = 0.043）。
本研究中的 GST 基因多态性频率与其他欧洲白种人群一致。基于本研究，GST 基因的变异性不是中欧人群 CRC 易感性的主要决定因素。
