DNA与小牛胸腺DNA螺旋去稳定蛋白相互作用诱导的DNA碱基暴露。

Exposure of DNA bases induced by the interaction of DNA and calf thymus DNA helix-destabilizing protein.

作者信息

Kohwi-Shigematsu T, Enomoto T, Yamada M A, Nakanishi M, Tsuboi M

出版信息

Proc Natl Acad Sci U S A. 1978 Oct;75(10):4689-93. doi: 10.1073/pnas.75.10.4689.

DOI:10.1073/pnas.75.10.4689

PMID:216994

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC336185/

Abstract

The reaction of chloroacetaldehyde with adenine bases in DNA to give a fluorescent product was used to study the availability to intermolecular reaction of positions 1 and 6 of adenine in DNA complexes with calf thymus DNA helix-destabilizing protein. No inhibition of this reaction was observed when heat-denatured DNA was complexed with the protein at a protein/DNA weight ratio of 10:1, compared to free DNA. On the contrary, the same reaction was inhibited markedly for denatured DNA in the presence of calf thymus histone HI at protein/DNA weight ratio of 2:1. Furthermore, the exchange rate for hydrogens of amino and imide groups of DNA bases in DNA strands with deuterium in the solvent was totally unaffected upon complexing of DNA with the DNA helix-destabilizing protein as examined by stopped-flow ultraviolet spectroscopy. These results indicate that the DNA helix-destabilizing protein forms a complex with single-stranded DNA, leaving DNA bases uncovered by the protein. The fluorescence intensity of DNA pretreated with chloroacetaldehyde was amplified by nearly 3-fold upon addition of the DNA helix-destabilizing protein. The possibility of "unstacking" of DNA bases induced by the protein is discussed.

摘要

利用氯乙醛与DNA中的腺嘌呤碱基反应生成荧光产物，来研究在与小牛胸腺DNA螺旋去稳定蛋白形成的DNA复合物中，腺嘌呤第1位和第6位发生分子间反应的可能性。与游离DNA相比，当热变性DNA与蛋白以蛋白/DNA重量比为10:1复合时，未观察到该反应受到抑制。相反，在小牛胸腺组蛋白H1存在下，当蛋白/DNA重量比为2:1时，变性DNA的相同反应受到明显抑制。此外，通过停流紫外光谱法检测发现，DNA链中DNA碱基的氨基和亚氨基上的氢与溶剂中的氘的交换速率在DNA与DNA螺旋去稳定蛋白复合后完全不受影响。这些结果表明，DNA螺旋去稳定蛋白与单链DNA形成复合物，使DNA碱基未被蛋白覆盖。加入DNA螺旋去稳定蛋白后，用氯乙醛预处理的DNA的荧光强度增加了近3倍。文中讨论了该蛋白诱导DNA碱基“解堆积”的可能性。

相似文献

Exposure of DNA bases induced by the interaction of DNA and calf thymus DNA helix-destabilizing protein.DNA与小牛胸腺DNA螺旋去稳定蛋白相互作用诱导的DNA碱基暴露。

Proc Natl Acad Sci U S A. 1978 Oct;75(10):4689-93. doi: 10.1073/pnas.75.10.4689.

A correlation between the secondary structure of DNA and the reactivity of adenine residues with chloroacetaldehyde.

J Biochem. 1977 Jun;81(6):1699-703. doi: 10.1093/oxfordjournals.jbchem.a131629.

HIV-1 nucleocapsid protein as a nucleic acid chaperone: spectroscopic study of its helix-destabilizing properties, structural binding specificity, and annealing activity.HIV-1核衣壳蛋白作为核酸伴侣：对其螺旋去稳定特性、结构结合特异性及退火活性的光谱学研究

J Mol Biol. 2002 May 3;318(3):749-64. doi: 10.1016/S0022-2836(02)00043-8.

The conformation of the complex of the helix destabilizing protein GP32 of bacteriophage T4 and single stranded DNA.

J Biomol Struct Dyn. 1985 Feb;2(4):821-9. doi: 10.1080/07391102.1985.10506326.

Raman spectroscopy of DNA-metal complexes. II. The thermal denaturation of DNA in the presence of Sr2+, Ba2+, Mg2+, Ca2+, Mn2+, Co2+, Ni2+, and Cd2+.DNA-金属络合物的拉曼光谱。II. Sr2+、Ba2+、Mg2+、Ca2+、Mn2+、Co2+、Ni2+和Cd2+存在下DNA的热变性

Biophys J. 1995 Dec;69(6):2623-41. doi: 10.1016/S0006-3495(95)80133-5.

A helix-destabilizing protein substrate devoid of heterocyclic bases.

Biochem Biophys Res Commun. 1981 May 29;100(2):760-8. doi: 10.1016/s0006-291x(81)80240-9.

On the thermodynamics and kinetics of the cooperative binding of bacteriophage T4-coded gene 32 (helix destabilizing) protein to nucleic acid lattices.关于噬菌体T4编码的基因32（解螺旋）蛋白与核酸晶格协同结合的热力学和动力学

Biophys J. 1980 Oct;32(1):403-18. doi: 10.1016/S0006-3495(80)84964-2.

Nucleic acid helix-coil transitions mediated by helix-unwinding proteins from calf thymus.小牛胸腺解旋蛋白介导的核酸螺旋-卷曲转变

J Biol Chem. 1976 Apr 10;251(7):2133-41.

Amino acid sequence of the UP1 calf thymus helix-destabilizing protein and its homology to an analogous protein from mouse myeloma.小牛胸腺解螺旋蛋白UP1的氨基酸序列及其与小鼠骨髓瘤中类似蛋白的同源性。

Proc Natl Acad Sci U S A. 1985 Sep;82(17):5666-70. doi: 10.1073/pnas.82.17.5666.

E. coli single strand binding protein: a new look at helix-destabilizing proteins.

Trends Biochem Sci. 1988 Jul;13(7):250-5.

引用本文的文献

Hydrogen-deuterium exchange studies of proteins and nucleic acids.蛋白质和核酸的氢-氘交换研究

Biophys J. 1980 Oct;32(1):621-3. doi: 10.1016/S0006-3495(80)84995-2.

Binding of RecA protein to single-stranded nucleic acids: spectroscopic studies using fluorescent polynucleotides.RecA蛋白与单链核酸的结合：使用荧光多核苷酸的光谱学研究。

EMBO J. 1983;2(12):2247-51. doi: 10.1002/j.1460-2075.1983.tb01730.x.

Detection of an altered DNA conformation at specific sites in chromatin and supercoiled DNA.检测染色质和超螺旋DNA中特定位点的DNA构象改变。

Proc Natl Acad Sci U S A. 1983 Jul;80(14):4389-93. doi: 10.1073/pnas.80.14.4389.

The structure of a DNA unwinding protein and its complexes with oligodeoxynucleotides by x-ray diffraction.通过X射线衍射解析DNA解旋蛋白的结构及其与寡脱氧核苷酸的复合物。

Biophys J. 1980 Oct;32(1):155-73. doi: 10.1016/S0006-3495(80)84931-9.

Unusual conformational effect exerted by Z-DNA upon its neighboring sequences.

Proc Natl Acad Sci U S A. 1987 Apr;84(8):2223-7. doi: 10.1073/pnas.84.8.2223.

Magnesium ion-dependent triple-helix structure formed by homopurine-homopyrimidine sequences in supercoiled plasmid DNA.超螺旋质粒DNA中由同型嘌呤-同型嘧啶序列形成的镁离子依赖性三螺旋结构。

Proc Natl Acad Sci U S A. 1988 Jun;85(11):3781-5. doi: 10.1073/pnas.85.11.3781.

Cationic metal-specific structures adopted by the poly(dG) region and the direct repeats in the chicken adult beta A globin gene promoter.

Nucleic Acids Res. 1989 Jun 26;17(12):4493-502. doi: 10.1093/nar/17.12.4493.

Detection of triple-helix related structures adopted by poly(dG)-poly(dC) sequences in supercoiled plasmid DNA.超螺旋质粒DNA中聚（dG）-聚（dC）序列所采用的三螺旋相关结构的检测。

Nucleic Acids Res. 1991 Aug 11;19(15):4267-71. doi: 10.1093/nar/19.15.4267.

本文引用的文献

Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割

Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.

Stimulation of T4 bacteriophage DNA polymerase by the protein product of T4 gene 32.T4基因32的蛋白质产物对T4噬菌体DNA聚合酶的刺激作用。

J Mol Biol. 1971 Nov 28;62(1):39-52. doi: 10.1016/0022-2836(71)90129-x.

Physical studies of chloroacetaldehyde labelled fluorescent DNA.氯乙醛标记荧光DNA的物理研究。

Biochem Biophys Res Commun. 1973 Feb 5;50(3):879-85. doi: 10.1016/0006-291x(73)91327-2.

Purification and properties of the Escherichia coli deoxyribonucleic acid-unwinding protein. Effects on deoxyribonucleic acid synthesis in vitro.大肠杆菌脱氧核糖核酸解旋蛋白的纯化及性质。对体外脱氧核糖核酸合成的影响。

J Biol Chem. 1974 Oct 10;249(19):6090-8.

DNA synthesis on a double-stranded DNA template by the T4 bacteriophage DNA polymerase and the T4 gene 32 DNA unwinding protein.T4噬菌体DNA聚合酶和T4基因32解旋蛋白在双链DNA模板上进行DNA合成。

J Biol Chem. 1974 Sep 10;249(17):5668-76.

A DNA-binding protein induced by bacteriophage T7.一种由噬菌体T7诱导产生的DNA结合蛋白。

Proc Natl Acad Sci U S A. 1973 Jun;70(6):1846-50. doi: 10.1073/pnas.70.6.1846.

A DNA-unwinding protein isolated from Escherichia coli: its interaction with DNA and with DNA polymerases.从大肠杆菌中分离出的一种DNA解旋蛋白：它与DNA及DNA聚合酶的相互作用。

Proc Natl Acad Sci U S A. 1972 Dec;69(12):3537-41. doi: 10.1073/pnas.69.12.3537.

Self-association of gene-32 protein of bacteriophage T4.噬菌体T4基因32蛋白的自我缔合

Proc Natl Acad Sci U S A. 1972 Sep;69(9):2741-4. doi: 10.1073/pnas.69.9.2741.

Fluorescent adenosine and cytidine derivatives.荧光腺苷和胞苷衍生物

Biochem Biophys Res Commun. 1972 Jan 31;46(2):597-604. doi: 10.1016/s0006-291x(72)80181-5.

Chloroacetaldehyde-modified dinucleoside phosphates. Dynamic fluorescence quenching and quenching due to intramolecular complexation.氯乙醛修饰的二核苷磷酸。动态荧光猝灭及分子内络合导致的猝灭。

Biochemistry. 1974 Nov 19;13(24):4869-78. doi: 10.1021/bi00721a001.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型，支持多种主流文档格式。