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Z-DNA 对其相邻序列施加的异常构象效应。

Unusual conformational effect exerted by Z-DNA upon its neighboring sequences.

作者信息

Kohwi-Shigematsu T, Manes T, Kohwi Y

出版信息

Proc Natl Acad Sci U S A. 1987 Apr;84(8):2223-7. doi: 10.1073/pnas.84.8.2223.

DOI:10.1073/pnas.84.8.2223
PMID:3470786
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC304621/
Abstract

Supercoiled plasmid DNA harboring an insert of (dG-dC)16, a sequence known to form Z-DNA upon negative supercoiling, was reacted with chloroacetaldehyde. Chloroacetaldehyde, like bromoacetaldehyde, was found to be a specific probe for detecting unpaired DNA bases in supercoiled plasmid DNA. Under torsional stress (at bacterial superhelical density), chloroacetaldehyde reacted at multiple discrete regions within the neighboring sequences of the (dG-dC)16 insert. When the plasmid population was considered as a whole, the distribution of the chemically reactive bases exhibited a pattern of inversion symmetry with the center of inversion in the middle of the (dG-dC)16 insert. However, when a single supercoiled plasmid molecule was considered, chloroacetaldehyde reacted with only one of the neighboring sequences, either 5' or 3' of the (dG-dC)16 insert, but not with both. The possibility that the supercoiled plasmid DNA is in equilibrium with these two structural forms is discussed.

摘要

携带(dG-dC)16插入片段的超螺旋质粒DNA，该序列在负超螺旋时已知会形成Z-DNA，使其与氯乙醛反应。氯乙醛与溴乙醛一样，被发现是检测超螺旋质粒DNA中未配对DNA碱基的特异性探针。在扭转应力下（处于细菌超螺旋密度），氯乙醛在(dG-dC)16插入片段相邻序列内的多个离散区域发生反应。当将整个质粒群体考虑在内时，化学反应性碱基的分布呈现出以(dG-dC)16插入片段中间为反转中心的反转对称模式。然而，当考虑单个超螺旋质粒分子时，氯乙醛仅与(dG-dC)16插入片段5'或3'端的相邻序列之一发生反应，而不是与两者都反应。文中讨论了超螺旋质粒DNA与这两种结构形式处于平衡状态的可能性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d839/304621/54401e9b15d6/pnas00273-0123-e.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d839/304621/9edae825c3d3/pnas00273-0122-a.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d839/304621/a05a18d224e2/pnas00273-0123-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d839/304621/5ced57ab8f34/pnas00273-0123-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d839/304621/59c8f20a9f6c/pnas00273-0123-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d839/304621/d40bac0614c1/pnas00273-0123-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d839/304621/54401e9b15d6/pnas00273-0123-e.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d839/304621/9edae825c3d3/pnas00273-0122-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d839/304621/65e7271c41a5/pnas00273-0122-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d839/304621/29965733e637/pnas00273-0122-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d839/304621/d7f9dbc2ebc2/pnas00273-0122-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d839/304621/a05a18d224e2/pnas00273-0123-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d839/304621/5ced57ab8f34/pnas00273-0123-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d839/304621/59c8f20a9f6c/pnas00273-0123-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d839/304621/d40bac0614c1/pnas00273-0123-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d839/304621/54401e9b15d6/pnas00273-0123-e.jpg

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1
Unusual conformational effect exerted by Z-DNA upon its neighboring sequences.Z-DNA 对其相邻序列施加的异常构象效应。
Proc Natl Acad Sci U S A. 1987 Apr;84(8):2223-7. doi: 10.1073/pnas.84.8.2223.
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引用本文的文献

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本文引用的文献

1
Negatively supercoiled plasmids contain left-handed Z-DNA segments as detected by specific antibody binding.通过特异性抗体结合检测发现，负超螺旋质粒含有左手Z-DNA片段。
Cell. 1982 Dec;31(2 Pt 1):309-18. doi: 10.1016/0092-8674(82)90124-6.
2
Junction between Z and B conformations in a DNA restriction fragment: evaluation by Raman spectroscopy.DNA限制片段中Z构象与B构象之间的连接：通过拉曼光谱法进行评估
Proc Natl Acad Sci U S A. 1982 Apr;79(8):2549-53. doi: 10.1073/pnas.79.8.2549.
3
Flipping of cloned d(pCpG)n.d(pCpG)n DNA sequences from right- to left-handed helical structure by salt, Co(III), or negative supercoiling.
超螺旋质粒DNA中由同型嘌呤-同型嘧啶序列形成的镁离子依赖性三螺旋结构。
Proc Natl Acad Sci U S A. 1988 Jun;85(11):3781-5. doi: 10.1073/pnas.85.11.3781.
4
Mung bean nuclease exhibits a generalized gene-excision activity upon purified Plasmodium falciparum genomic DNA.绿豆核酸酶对纯化的恶性疟原虫基因组DNA表现出普遍的基因切除活性。
Nucleic Acids Res. 1988 Jul 25;16(14B):6883-96. doi: 10.1093/nar/16.14.6883.
5
Reaction conditions affect the specificity of bromoacetaldehyde as a probe for DNA cruciforms and B-Z junctions.
Nucleic Acids Res. 1987 Sep 11;15(17):6917-35. doi: 10.1093/nar/15.17.6917.
6
Non-contiguous regions of Z-DNA in a DNA dodecamer.DNA十二聚体中Z-DNA的非连续区域。
Nucleic Acids Res. 1989 Oct 11;17(19):7923-44. doi: 10.1093/nar/17.19.7923.
7
Cationic metal-specific structures adopted by the poly(dG) region and the direct repeats in the chicken adult beta A globin gene promoter.鸡成年βA珠蛋白基因启动子中多聚（dG）区域和直接重复序列所采用的阳离子金属特异性结构。
Nucleic Acids Res. 1989 Jun 26;17(12):4493-502. doi: 10.1093/nar/17.12.4493.
8
Distortions induced in double-stranded oligonucleotides by the binding of cis- or trans-diammine-dichloroplatinum(II) to the d(GTG) sequence.顺式或反式二氯二氨铂（II）与d(GTG)序列结合诱导双链寡核苷酸产生的畸变。
Nucleic Acids Res. 1990 Aug 11;18(15):4395-400. doi: 10.1093/nar/18.15.4395.
9
The influence of an alternate template conformation on elongating phage T7 RNA polymerase.交替模板构象对噬菌体T7 RNA聚合酶延伸的影响。
Nucleic Acids Res. 1991 Oct 11;19(19):5301-6. doi: 10.1093/nar/19.19.5301.
10
Detection of triple-helix related structures adopted by poly(dG)-poly(dC) sequences in supercoiled plasmid DNA.超螺旋质粒DNA中聚（dG）-聚（dC）序列所采用的三螺旋相关结构的检测。
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通过盐、钴（III）或负超螺旋使克隆的d(pCpG)n.d(pCpG)n DNA序列从右手螺旋结构转变为左手螺旋结构。
Proc Natl Acad Sci U S A. 1982 Aug;79(15):4560-4. doi: 10.1073/pnas.79.15.4560.
4
Facile transition of poly[d(TG) x d(CA)] into a left-handed helix in physiological conditions.在生理条件下，聚[d(TG)x d(CA)]能轻松转变为左手螺旋。
Nature. 1983 Apr 14;302(5909):632-4. doi: 10.1038/302632a0.
5
The sequence (dC-dA)n X (dG-dT)n forms left-handed Z-DNA in negatively supercoiled plasmids.序列（dC-dA）n×（dG-dT）n在负超螺旋质粒中形成左手Z-DNA。
Proc Natl Acad Sci U S A. 1983 Apr;80(7):1821-5. doi: 10.1073/pnas.80.7.1821.
6
The chemistry and biology of left-handed Z-DNA.左手螺旋Z-DNA的化学与生物学
Annu Rev Biochem. 1984;53:791-846. doi: 10.1146/annurev.bi.53.070184.004043.
7
Detection of an altered DNA conformation at specific sites in chromatin and supercoiled DNA.检测染色质和超螺旋DNA中特定位点的DNA构象改变。
Proc Natl Acad Sci U S A. 1983 Jul;80(14):4389-93. doi: 10.1073/pnas.80.14.4389.
8
Structural perturbation in supercoiled DNA: hypersensitivity to modification by a single-strand-selective chemical reagent conferred by inverted repeat sequences.超螺旋DNA中的结构扰动：反向重复序列赋予对单链选择性化学试剂修饰的超敏性。
Nucleic Acids Res. 1983 May 25;11(10):3097-112. doi: 10.1093/nar/11.10.3097.
9
A dominant role for DNA secondary structure in forming hypersensitive structures in chromatin.DNA二级结构在染色质中形成超敏感结构方面起主导作用。
Cell. 1983 Apr;32(4):1191-203. doi: 10.1016/0092-8674(83)90302-1.
10
Left-handed Z-DNA is induced by supercoiling in physiological ionic conditions.在生理离子条件下，超螺旋可诱导左手Z-DNA的形成。
Nature. 1982 Sep 23;299(5881):312-6. doi: 10.1038/299312a0.