Harvard Medical School, Department of Systems Biology, 200 Longwood Avenue, Boston, MA 02115, USA.
Science. 2011 Jul 22;333(6041):470-4. doi: 10.1126/science.1206938. Epub 2011 Jun 23.
The rules of nucleic acid base-pairing have been used to construct nanoscale architectures and organize biomolecules, but little has been done to apply this technology in vivo. We designed and assembled multidimensional RNA structures and used them as scaffolds for the spatial organization of bacterial metabolism. Engineered RNA modules were assembled into discrete, one-dimensional, and two-dimensional scaffolds with distinct protein-docking sites and used to control the spatial organization of a hydrogen-producing pathway. We increased hydrogen output as a function of scaffold architecture. Rationally designed RNA assemblies can thus be used to construct functional architectures in vivo.
核酸碱基配对规则已被用于构建纳米级结构和组织生物分子，但在体内应用该技术的研究还很少。我们设计并组装了多维 RNA 结构，并将其用作细菌代谢空间组织的支架。设计的 RNA 模块被组装成离散的、一维的和二维的支架，具有独特的蛋白质结合位点，并用于控制产氢途径的空间组织。我们使氢气的产量随着支架结构的变化而变化。因此，通过合理设计的 RNA 组装可以在体内构建功能性结构。
