基于活性的蛋白质谱分析（ABPP）以及通过多维蛋白质鉴定技术质谱法进行的点击化学（CC）-ABPP
Activity-Based Protein Profiling (ABPP) and Click Chemistry (CC)-ABPP by MudPIT Mass Spectrometry.
作者信息
Speers Anna E, Cravatt Benjamin F
机构信息
The Skaggs Institute for Chemical Biology and Department of Physiological Chemistry, The Scripps Research Institute, La Jolla, CA.
出版信息
Curr Protoc Chem Biol. 2009;1:29-41. doi: 10.1002/9780470559277.ch090138.
Abstract
Activity-based protein profiling (ABPP) is a chemical proteomic method for functional interrogation of enzymes within complex proteomes. This Unit presents a protocol for in vitro and in vivo labeling using ABPP and Click Chemistry (CC)-ABPP probes for in-depth profiling using the Multi-dimensional Protein Identification Technology (MudPIT) analysis platform.
摘要
基于活性的蛋白质谱分析（ABPP）是一种用于在复杂蛋白质组中对酶进行功能研究的化学蛋白质组学方法。本单元介绍了一种使用ABPP和点击化学（CC）-ABPP探针进行体外和体内标记的方案，用于使用多维蛋白质鉴定技术（MudPIT）分析平台进行深入的谱分析。
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