Department of Clinical and Biological Sciences, University of Turin, Reg. Gonzole 10, Orbassano, TO, Italy.
Calcif Tissue Int. 2011 Aug;89(2):163-71. doi: 10.1007/s00223-011-9503-3. Epub 2011 Jun 24.
Fracture consolidation is a crucial goal to achieve as early as possible, but pharmacological stimulation has been neglected so far. Teriparatide has been considered for this purpose for its anabolic properties. We set up a murine model of closed tibial fracture on which different doses of teriparatide were tested. Closed fracture treatment avoids any bias introduced by surgical manipulations. Teriparatide's effect on callus formation was monitored during the first 4 weeks from fracture. Callus evolution was determined by histomorphometric and microhardness assessment. Daily administration of 40 μg/kg of teriparatide accelerated callus mineralization from day 9 onward without significant increase of sizes, and at day 15 the microhardness properties of treated callus were similar to those of bone tissue. Teriparatide considerably improved callus consolidation in the very early phases of bone healing.
骨折愈合是尽早实现的关键目标，但迄今为止，药物刺激一直被忽视。特立帕肽因其具有合成代谢特性而被认为具有这种作用。我们建立了一种闭合性胫骨骨折的小鼠模型，在该模型上测试了不同剂量的特立帕肽。闭合性骨折治疗避免了任何由手术操作引入的偏差。在骨折后 4 周内监测特立帕肽对骨痂形成的影响。通过组织形态计量学和显微硬度评估来确定骨痂的演变。每天给予 40μg/kg 的特立帕肽可从第 9 天开始加速骨痂矿化，而大小无明显增加，在第 15 天，治疗性骨痂的显微硬度特性与骨组织相似。特立帕肽在骨愈合的早期阶段显著改善了骨痂的愈合。
