通过单量子点追踪确定FcεRI的扩散动力学。

Determining FcεRI diffusional dynamics via single quantum dot tracking.

作者信息

Lidke Diane S, Low-Nam Shalini T, Cutler Patrick J, Lidke Keith A

机构信息

Department of Pathology and Cancer Research and Treatment Center, University of New Mexico, Albuquerque, NM, USA.

出版信息

Methods Mol Biol. 2011;748:121-32. doi: 10.1007/978-1-61779-139-0_8.

DOI:10.1007/978-1-61779-139-0_8

PMID:21701970

Abstract

Single-particle tracking (SPT) using fluorescent quantum dots (QDs) provides high-resolution spatial-temporal information on receptor dynamics that cannot be obtained through traditional biochemical techniques. In particular, the high brightness and photostability of QDs make them ideal probes for SPT on living cells. We have shown that QD-labeled IgE can be used to characterize the dynamics of the high-affinity IgE Receptor. Here, we describe protocols for (1) coupling QDs to IgE, (2) tracking individual QD-bound receptors, and (3) analyzing one- and two-color tracking data.

摘要

使用荧光量子点（QD）的单粒子追踪（SPT）可提供关于受体动力学的高分辨率时空信息，而这是传统生化技术无法获得的。特别是，量子点的高亮度和光稳定性使其成为活细胞上SPT的理想探针。我们已经表明，量子点标记的IgE可用于表征高亲和力IgE受体的动力学。在此，我们描述了以下方案：（1）将量子点与IgE偶联，（2）追踪单个量子点结合的受体，以及（3）分析单色和双色追踪数据。