一种参与洋葱伯克霍尔德菌中4-甲基邻苯二甲酸酯和4-羟基间苯二甲酸酯降解的分解代谢质粒。

A catabolic plasmid involved in 4-methyl-o-phthalate and 4-hydroxy-iso-phthalate degradation in Pseudomonas cepacia.

作者信息

Saint C P, Ribbons D W

机构信息

School of Pure and Applied Biology, University of Wales College of Cardiff, U.K.

出版信息

FEMS Microbiol Lett. 1990 Jun 1;57(3):323-8. doi: 10.1016/0378-1097(90)90088-8.

DOI:10.1016/0378-1097(90)90088-8

PMID:2170232

Abstract

Genes involved in 4-methyl-o-phthalate and 4-hydroxy-iso-phthalate catabolism reside on a 226-232 kbp catabolic plasmid termed MOP. This was confirmed by transformation and conjugation into an isogenic heat-cured (MOP-) derivative of the wild-type isolate, identified and termed Pseudomonas cepacia Pc701. Transformation confirmed the presence of Tn1 in MOP derived from Pc704, a mutant deficient in 4-methyl-o-phthalate catabolism. pCS1, a recombinant plasmid bearing MOP DNA, complemented MOP::Tn1 restoring the ability of Pc704 to grow on 4-methyl-o-phthalate. DNA-DNA hybridization using pCS1 as probe confirmed that loss of 4-methyl-o-phthalate catabolism by Pc704 was the result of Tn1 insertion into a 2.1 kbp HindIII fragment of MOP.

摘要

参与4-甲基邻苯二甲酸酯和4-羟基间苯二甲酸酯分解代谢的基因位于一个226 - 232 kbp的分解代谢质粒上，该质粒称为MOP。通过将其转化和接合到野生型菌株的同基因热治愈（MOP-）衍生物中得到证实，该衍生物被鉴定并命名为洋葱伯克霍尔德菌Pc701。转化证实了来自Pc704（一种4-甲基邻苯二甲酸酯分解代谢缺陷型突变体）的MOP中存在Tn1。携带MOP DNA的重组质粒pCS1补充了MOP::Tn1，恢复了Pc704在4-甲基邻苯二甲酸酯上生长的能力。以pCS1为探针的DNA-DNA杂交证实，Pc704丧失4-甲基邻苯二甲酸酯分解代谢能力是由于Tn1插入到MOP的一个2.1 kbp HindIII片段中所致。