钠钾ATP酶的调节。II. 编码β2亚基的大鼠NKAB2基因5'侧翼区的克隆与分析。

Regulation of Na+,K(+)-ATPase. II. Cloning and analysis of the 5'-flanking region of the rat NKAB2 gene encoding the beta 2 subunit.

作者信息

Kawakami K, Okamoto H, Yagawa Y, Nagano K

机构信息

Department of Biology, Jichi Medical School, Tochigi, Japan.

出版信息

Gene. 1990 Jul 16;91(2):271-4. doi: 10.1016/0378-1119(90)90099-d.

DOI:10.1016/0378-1119(90)90099-d

PMID:2170236

Abstract

We cloned a 14.5-kb fragment of genomic DNA spanning at least the first two exons of the rat Na+,K(+)-ATPase (EC 3.6.1.3) beta 2 subunit-encoding gene and 5.5 kb of the 5'-flanking region. The transcription start point (tsp) is located 595 bp upstream from the start codon. The tsp was identical for adult rat brain and spleen, both of which produce the beta 2 isoform. The TATA sequence was found 29 bp upstream from the tsp and Sp1-binding sites at nucleotides (nt) -55 and -147. In addition, multiple consensus binding sites for a wide variety of regulatory proteins were present throughout the upstream and downstream tsp-flanking regions. Conserved sequence elements which may serve for coordinated expression of the alpha and beta subunits were found in the nt sequence of the 5'-flanking region.

摘要

我们克隆了一段14.5kb的基因组DNA片段，该片段至少跨越大鼠Na⁺,K⁺-ATP酶（EC 3.6.1.3）β2亚基编码基因的前两个外显子以及5.5kb的5'侧翼区域。转录起始点（tsp）位于起始密码子上游595bp处。成年大鼠脑和脾中的转录起始点相同，二者均产生β2同工型。TATA序列位于转录起始点上游29bp处，在核苷酸（nt）-55和-147处存在Sp1结合位点。此外，在转录起始点上下游侧翼区域中均存在多种调节蛋白的多个共有结合位点。在5'侧翼区域的核苷酸序列中发现了可能用于α和β亚基协同表达的保守序列元件。