Laboratoire de Biologie des Ligneux et des Grandes Cultures (LBLGC), UPRES EA 1207, Antenne Scientifique Universitaire de Chartres (ASUC), Université d'Orléans, Chartres, France.
J Agric Food Chem. 2011 Aug 10;59(15):8101-7. doi: 10.1021/jf201410p. Epub 2011 Jul 15.
The lignans podophyllotoxin and deoxypodophyllotoxin are secondary metabolites with potent pharmaceutical applications in cancer therapy. However, the supply of podophyllotoxin from its current natural source, Podophyllum hexandrum, is becoming increasingly problematic, and alternative sources are therefore urgently needed. So far, podophyllotoxin and deoxypodophyllotoxin have been found in some Juniperus species, although at low levels in most cases. Moreover, extraction protocols deserve optimization. This study aimed at developing and validating an efficient extraction protocol of podophyllotoxin and deoxypodophyllotoxin from Juniperus species and applying it to 13 Juniperus species, among which some had never been previously analyzed. Juniperus bermudiana was used for the development and validation of an extraction protocol for podophyllotoxin and deoxypodophyllotoxin allowing extraction yields of up to 22.6 mg/g DW of podophyllotoxin and 4.4 mg/g DW deoxypodophyllotoxin, the highest values found in leaf extract of Juniperus. The optimized extraction protocol and HPLC separation from DAD or MS detections were established and validated to investigate podophyllotoxin and deoxypodophyllotoxin contents in aerial parts of 12 other Juniperus species. This allowed either higher yields to be obtained in some species reported to contain these two compounds or the occurrence of these compounds in some other species to be reported for the first time. This efficient protocol allows effective extraction of podophyllotoxin and deoxypodophyllotoxin from aerial parts of Juniperus species, which could therefore constitute interesting alternative sources of these valuable metabolites.
木脂素鬼臼毒素和脱氧鬼臼毒素是具有强大药用应用价值的次生代谢产物，可用于癌症治疗。然而，从目前的天然来源——尼泊尔鬼臼（Podophyllum hexandrum）中提取鬼臼毒素的供应正变得越来越成问题，因此急需寻找替代来源。迄今为止，已在一些杜松属（Juniperus）物种中发现了鬼臼毒素和脱氧鬼臼毒素，尽管在大多数情况下含量都很低。此外，提取方案需要优化。本研究旨在开发和验证一种从杜松属物种中高效提取鬼臼毒素和脱氧鬼臼毒素的方法，并将其应用于 13 种杜松属物种，其中一些物种以前从未进行过分析。百慕大杜松（Juniperus bermudiana）被用于开发和验证一种用于提取鬼臼毒素和脱氧鬼臼毒素的方法，该方法可使鬼臼毒素的提取产率高达 22.6 mg/g DW，脱氧鬼臼毒素的提取产率为 4.4 mg/g DW，这是杜松属植物叶提取物中发现的最高值。建立并验证了优化的提取方案和 HPLC 分离方法（基于 DAD 或 MS 检测），以调查 12 种其他杜松属物种地上部分中鬼臼毒素和脱氧鬼臼毒素的含量。这使得在一些报告含有这两种化合物的物种中可以获得更高的产率，或者在一些其他物种中首次报道了这两种化合物的存在。这种高效的方法可以有效地从杜松属植物的地上部分中提取鬼臼毒素和脱氧鬼臼毒素，因此它们可能成为这些有价值代谢物的有趣替代来源。
